Human BMP-2 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant BMP-2 based upon sequence from: Human
Host: QP5363 protein expressed in E. coli or HEK293 cells (make selection above to chose the host of the protein).
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5363.
Bioactivity Data: BioActivity: E. coli synthesized BMP-2 shows bioactivity at a concentration of 250 ng/mL in the Alkaline phosphatase activity induced in ATDC-5 cells assay. The bioactivity acceptance criteria is set at 4 x 10^3 units/mg. The more potent HEK293 derived BMP2 has a specific activity of less than 40 ng/ml in ATDC-5 cell induced alk phos activity.
Monomer or Dimer:Glycosylated Homodimer
Amino Acid Sequence: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: We recommend reconstitution of human BMP-2 in sterile 4mM HCl containing 0.1% endotoxin-free recombinant HSA.
Concentration of Human BMP-2 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: Lyophilized from a sterile filtered (0.22 um) 2x PBS solution.
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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