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Recombinant Human AKR1C1 Protein

$ 89.00$ 2,250.00
Please Select A Product To View The SKU.

SKU: QP10513
Species: Human
Applications: Bioactive
Available Tags: His
Available Hosts: E. Coli
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 343

Size
Tag Clear selection
Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10513-5ug
Recombinant Human AKR1C1 General Information
Alternate Names
DDH1, DDH, HAKRC, 20-alpha-HSD, DD1/DD2, HBAB, C9, DD1, H-37, MBAB, MGC8954, 2-ALPHA-HSD, AKR1C1, Aldo-keto reductase family 1 member C1, 20-alpha-hydroxysteroid dehydrogenase, Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, Indanol dehydrogenase, Dihydrodiol dehydrogenase 1/2, Chlordecone reductase homolog HAKRC, High-affinity hepatic bile acid-binding protein.
Molecular Weight
38.9 kDa
Curated Database and Bioinformatic Data
UniProt ID(s)Q04828
General Description of Recombinant Human AKR1C1.
Human AKR1C1 fused to a 20 amino acid His Tag at N-terminus produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 343 amino acids (1-323 AA ) and having a molecular mass of 38.9 kDa. The AKR1C1 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Human AKR1C1 Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant AKR1C1 based upon sequence from Human
Host: QP10513 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of AKR1C1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10513.
Bioactivity Data: Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25°C.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels:
Buffer: The AKR1C1 protein solution (0.5 mg/ml) contains 20mM Tris-HCl pH 8.0, 1mM DTT and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear colorless solution.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human AKR1C1 Protein