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Recombinant Mouse GAPDH Protein

$ 89.00$ 5,200.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP10624
Species: Mouse
Applications: Bioactive
Available Tags: His
Available Hosts: E. Coli
Purity: Greater than 95% as determined by SDS-PAGE.
Length (Amino Acids):

Size
Tag Clear selection
Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10624-2ug
Recombinant Mouse GAPDH General Information
GAPDH  protein 3D structural model from Catalog of Somatic Mutations in Cancer originally published in the paper COSMIC: somatic cancer genetics at high-resolution
Alternate Names
Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, Gapdh, Gapd, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, G3PD, GAPD, HEL-S-162eP.
Molecular Weight
38.2 kDa
Chromosomal Location
F2 on chromosome 6
Curated Database and Bioinformatic Data
Gene SymbolGapdh
Entrez Gene ID14433; 100042025
UniProt ID(s)P16858
COSMIC ID Link(s)Gapdh
KEGG Gene ID(s)mmu:100042025; mmu:14433
General Description of Recombinant Mouse GAPDH.
Mouse GAPDH produced in E. Coli is a single, non-glycosylated polypeptide chain containing 356 amino acids (1-333AA ) and having a molecular mass of 38.2kDa. GAPDH is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Mouse GAPDH Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant GAPDH based upon sequence from Mouse
Host: QP10624 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of GAPDH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10624.
Bioactivity Data: Specific activity is > 40 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVKVGVN GFGRIGRLVT RAAICSGKVE IVAINDPFID LNYMVYMFQY DSTHGKFNGT VKAENGKLVI NGKPITIFQE RDPTNIKWGE AGAEYVVEST GVFTTMEKAG AHLKGGAKRV IISAPSADAP MFVMGVNHEK YDNSLKIVSN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH AITATQKTVD GPSGKLWRDG RGAAQNIIPA STGAAKAVGK VIPELNGKLT GMAFRVPTPN VSVVDLTCRL EKPAKYDDIK KVVKQASEGP LKGILGYTED QVVSCDFNSN SHSSTFDAGA GIALNDNFVK LISWYDNEYG YSNRVVDLMA YMASKE
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels:
Buffer: GAPDH protein solution (0.25 mg/ml) containing Phosphate buffered saline (pH 7.4), 20% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Mouse GAPDH Protein