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Recombinant Mouse GLO1 Protein

$ 89.00$ 1,200.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP10645
Species: Mouse
Applications: Bioactive
Available Tags: His
Available Hosts: Insect
Purity: Greater than 95.0% as determined by SDS-PAGE.
Length (Amino Acids):

Size
Tag Clear selection
Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10645-2ug
Recombinant Mouse GLO1 General Information
GLO1  protein 3D structural model from Catalog of Somatic Mutations in Cancer originally published in the paper COSMIC: somatic cancer genetics at high-resolution
Alternate Names
Lactoylglutathione lyase, Aldoketomutase, Glyoxalase I, Glx I, Ketone-aldehyde mutase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase.
Molecular Weight
21.8 kDa
Chromosomal Location
A3.3 on chromosome 17
Curated Database and Bioinformatic Data
Gene SymbolGlo1
Entrez Gene ID109801
UniProt ID(s)Q9CPU0
COSMIC ID Link(s)Glo1
KEGG Gene ID(s)mmu:109801
General Description of Recombinant Mouse GLO1.
Mouse GLO1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 192 amino acids (1-184AA ) and having a molecular mass of 21.8kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). GLO1 is expressed with an 8 amino acid His tag at C-Terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Mouse GLO1 Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant GLO1 based upon sequence from Mouse
Host: QP10645 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of GLO1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10645.
Bioactivity Data: Specific activity is > 210 units/mg, and is defined as the amount of enzyme that will form 1.0 µmol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25C.
Amino Acid Sequence: MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTA WTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKI ATIILEHHHH HH
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels:
Buffer: GLO1 protein solution (0.5 mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Mouse GLO1 Protein