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Recombinant Ovine Prolactin Protein

$ 89.00$ 700.00
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SKU: QP10851
Species: Ovine
Applications: Bioactive
Available Tags: Untagged
Available Hosts: E. Coli
Purity: Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Length (Amino Acids):

Size
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Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10851-EC-10ug
Recombinant Ovine Prolactin General Information
Alternate Names
Mammotropin, Luteotropic hormone, Luteotropin, PRL.
Molecular Weight
23 kDa
Curated Database and Bioinformatic Data
UniProt ID(s)P01240
General Description of Recombinant Ovine Prolactin.
Prolactin Ovine Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 23 kDa. The Prolactin n is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Ovine Prolactin Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant Prolactin based upon sequence from Ovine
Host: QP10851 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Prolactin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
Amino Acid Sequence: MDSKGSAQKG SRLLLLLVVS NLLLCQGVVS TPVCPNGPGN CQVSLRDLFD RAVMVSHYIH NLSSEMFNEF DKRYAQGKGF ITMALNSCHT SSLPTPEDKE QAQQTHHEVL MSLILGLLRS WNDPLYHLVT EVRGMKGVPD AILSRAIEIE EENKRLLEGM EMIFGQVIPG AKETEPYPVW SGLPSLQTKD EDARHSAFYN LLHCLRRDSS KIDTYLKLLN CRIIYNNNC
Purity: Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Ovine Prolactin Protein: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Prolactin as a Reference Standard.
Endotoxin Levels:
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045mM NaHCO3.
Storage Conditions: Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Selected References

1.Prolactin Treatment Improves Engraftment and Function of Transplanted Pancreatic Islets.Publication:Published online before print December 18, 2008, doi: 10.1210/en.2008-1318 Endocrinology April 1, 2009 vol. 150 no. 4 1646-1653 .Link:http://endo.endojournals.org/content/150/4/1646.full2.ISLET ENDOTHELIAL CELLS AND PANCREATIC ?-CELL PROLIFERATION: STUDIES IN VITRO AND DURING PREGNANCY IN ADULT RATS.Publication:Endocrinology. First published January 26, 2006 as doi:10.1210/en.2005-0997.Link:http://endo.endojournals.org/content/early/2006/01/26/en.2005-0997.full.pdf3.Prolactin Treatment Improves Engraftment and Function of Transplanted Pancreatic Islets.Publication:Endocrinology. First published ahead of print December 18, 2008 as doi:10.1210/en.2008-1318.Link:http://endo.endojournals.org/content/early/2008/12/18/en.2008-1318.full.pdf4. Islet Endothelial Cells and Pancreatic -CellProliferation: Studies in Vitro and during Pregnancy in Adult Rats.Publication:Published online before print January 26, 2006, doi:10.1210/en.2005-0997 Endocrinology May 1, 2006vol. 147no. 52315-2324.Link:http://endo.endojournals.org/content/147/5/2315.full.pdf+html

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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