SDS-PAGE separation of QP7075 followed by commassie total protein stain results in a primary band consistent with reported data for DNA ligase. These data demonstrate Greater than 90% as determined by SDS-PAGE.
SDS-PAGE separation of QP7075 followed by commassie total protein stain results in a primary band consistent with reported data for DNA ligase. These data demonstrate Greater than 90% as determined by SDS-PAGE.

Recombinant E. coli DNA ligase Protein

$ 328.00$ 2,768.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP7075
Species: E. coli
Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Available Tags: His
Host: E. coli
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 671

Product NumberDescriptionPrice
QP7075-10ug Size: 10 ug, Tag: His $ 328.00
QP7075-50ug Size: 50 ug, Tag: His $ 458.00
QP7075-100ug Size: 100 ug, Tag: His $ 798.00
QP7075-200ug Size: 200 ug, Tag: His $ 1,298.00
QP7075-500ug Size: 500 ug, Tag: His $ 1,738.00
QP7075-1mg Size: 1 mg, Tag: His $ 2,768.00
Shipping Information
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

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Purity:

Bioactivity (test results eg. IU/ml):


SKU: QP7075-ec-50ug
Recombinant E. coli DNA ligase Protein General Information
Alternate Names
dnaL, b2411, ECK2406, JW2403, lig, lop, pdeC
Curated Database and Bioinformatic Data
Gene SymbolligA
Entrez Gene ID946885
RefSeq Protein Accession(s)NP_416906.1
RefSeq mRNA Accession(s)NC_000913.3, NZ_LN832404.1
UniProt ID(s)P15042
KEGG Gene ID(s)ecj:JW2403
General Description of Recombinant E. coli DNA ligase Protein.
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

E. coli DNA ligase Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant DNA ligase based upon sequence from: E. coli
Host: QP7075 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) DNA ligase, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP7075.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 - Ser671
Amino Acid Sequence: MESIEQQLTE LRTTLRHHEY LYHVMDAPEI PDAEYDRLMR ELRELETKHP ELITPDSPTQ RVGAAPLAAF SQIRHEVPML SLDNVFDEES FLAFNKRVQD RLKNNEKVTW CCELKLDGLA VSILYENGVL VSAATRGDGT TGEDITSNVR TIRAIPLKLH GENIPARLEV RGEVFLPQAG FEKINEDARR TGGKVFANPR NAAAGSLRQL DPRITAKRPL TFFCYGVGVL EGGELPDTHL GRLLQFKKWG LPVSDRVTLC ESAEEVLAFY HKVEEDRPTL GFDIDGVVIK VNSLAQQEQL GFVARAPRWA VAFKFPAQEQ MTFVRDVEFQ VGRTGAITPV ARLEPVHVAG VLVSNATLHN ADEIERLGLR IGDKVVIRRA GDVIPQVVNV VLSERPEDTR EVVFPTHCPV CGSDVERVEG EAVARCTGGL ICGAQRKESL KHFVSRRAMD VDGMGDKIID QLVEKEYVHT PADLFKLTAG KLTGLERMGP KSAQNVVNAL EKAKETTFAR FLYALGIREV GEATAAGLAA YFGTLEALEA ASIEELQKVP DVGIVVASHV HNFFAEESNR NVISELLAEG VHWPAPIVIN AEEIDSPFAG KTVVLTGSLS QMSRDDAKAR LVELGAKVAG SVSKKTDLVI AGEAAGSKLA KAQELGIEVI DEAEMLRLLG S
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli DNA ligase Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant E. coli DNA ligase Protein

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