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Recombinant Human HLA-DRB Protein

$ 448.00$ 1,938.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP7354
Species: Human
Available Tags: HIs-Myc
Host: E. coli
Purity: Greater than 80% as determined by SDS-PAGE.
Length (Amino Acids): 198

Product NumberDescriptionPrice
QP7354-ec-100ug Size: 100 ug, Tag: His-Myc $ 448.00
QP7354-ec-1mg Size: 1 mg, Tag: His-Myc $ 1,938.00
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7-10 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

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Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

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Purity:

Bioactivity (test results eg. IU/ml):


SKU: QP7354-ec-10ug
Recombinant Human HLA-DRB Protein (30-227aa) General Information
Curated Database and Bioinformatic Data
Gene SymbolHLA-DRB1
Ensemble Gene IDENSG00000196126
UniProt ID(s)P04229
UniGene ID(s)Hs.534322
HGNC ID(s)HGNC:4948
COSMIC ID Link(s)HLA-DRB1
General Description of Recombinant Human HLA-DRB Protein (30-227aa).
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading. (Microbial infection) Acts as a receptor for Epstein-Barr virus on lymphocytes.

Human HLA-DRB Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant HLA-DRB based upon sequence from: Human
Host: QP7354 protein expressed in E. coli.
Tag: HIs-Myc
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) HLA-DRB, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7354.
Bioactivity Data: Untested
Full Length? Extracellular Domain
Expression Region: Gly30 - Lys227
Amino Acid Sequence: GDTRPRFLWQ LKFECHFFNG TERVRLLERC IYNQEESVRF DSDVGEYRAV TELGRPDAEY WNSQKDLLEQ RRAAVDTYCR HNYGVGESFT VQRRVEPKVT VYPSKTQPLQ HHNLLVCSVS GFYPGSIEVR WFRNGQEEKA GVVSTGLIQN GDWTFQTLVM LETVPRSGEV YTCQVEHPSV TSPLTVEWRA RSESAQSK
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human HLA-DRB Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human HLA-DRB Protein