enQuire Bio » Products » Recombinant Protein » Recombinant E. coli Hygromycin-B 4-O-kinase Protein
SEQUEST analysis of LC MS/MS spectra obtained from a run with QP7397 identified a match between this protein and the spectra of a peptide sequence that matches a region of Hygromycin-B 4-O-kinase.
SEQUEST analysis of LC MS/MS spectra obtained from a run with QP7397 identified a match between this protein and the spectra of a peptide sequence that matches a region of Hygromycin-B 4-O-kinase.Additional SEQUEST analysis of the LC MS/MS spectra from QP7397 identified an additional between this protein and the spectra of another peptide sequence that matches a region of Hygromycin-B 4-O-kinase confirming successful recombinant synthesis.SDS-PAGE separation of QP7397 followed by commassie total protein stain results in a primary band consistent with reported data for Hygromycin-B 4-O-kinase. These data demonstrate Greater than 90% as determined by SDS-PAGE.

Recombinant E. coli Hygromycin-B 4-O-kinase Protein

$ 728.00 – $ 2,468.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP7397
Species: E. coli
Available Tags: His-SUMO, His
Host: E.coli, Yeast.
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 341

Product NumberDescriptionPrice
QP7397-ec-100ug Size: 100 ug, Tag: His-SUMO $ 728.00
QP7397-ec-1mg Size: 1 mg, Tag: His-SUMO $ 2,468.00
QP7397-ye-100ug Size: 100 ug, Tag: His $ 828.00
QP7397-ye-500ug Size: 500 ug, Tag: His $ 1,918.00
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7-10 days
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Datasheets and Documentation
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SKU: QP7397-ec-10ug
Recombinant E. coli Hygromycin-B 4-O-kinase Protein General Information
Alternate Names
PWD4IncI1_10
Curated Database and Bioinformatic Data
Gene Symbolhph
Entrez Gene ID13905269
RefSeq Protein Accession(s)WP_000742814.1
RefSeq mRNA Accession(s)NZ_NGVB01000039.1, NC_019061.1
UniProt ID(s)P00557
KEGG Gene ID(s)ag:CAA24743
General Description of Recombinant E. coli Hygromycin-B 4-O-kinase Protein.
The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related compounds such as dethyl analogs and destomycin.

E. coli Hygromycin-B 4-O-kinase Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant Hygromycin-B 4-O-kinase based upon sequence from: E. coli
Host: QP7397 protein expressed in E.coli, Yeast..
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Hygromycin-B 4-O-kinase, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7397.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 - Glu341
Amino Acid Sequence: MKKPELTATS VEKFLIEKFD SVSDLMQLSE GEESRAFSFD VGGRGYVLRV NSCADGFYKD RYVYRHFASA ALPIPEVLDI GEFSESLTYC ISRRAQGVTL QDLPETELPA VLQPVAEAMD AIAAADLSQT SGFGPFGPQG IGQYTTWRDF ICAIADPHVY HWQTVMDDTV SASVAQALDE LMLWAEDCPE VRHLVHADFG SNNVLTDNGR ITAVIDWSEA MFGDSQYEVA NIFFWRPWLA CMEQQTRYFE RRHPELAGSP RLRAYMLRIG LDQLYQSLVD GNFDDAAWAQ GRCDAIVRSG AGTVGRTQIA RRSAAVWTDG CVEVLADSGN RRPSTRPRAK E
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Hygromycin-B 4-O-kinase Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant E. coli Hygromycin-B 4-O-kinase Protein

https://enquirebio.com/wp-content/uploads/2018/01/QP7397-Escherichia-coli-strain-K12-Hygromycin-B-4-O-kinase-1-1-300x117.jpghttps://enquirebio.com/wp-content/uploads/2018/01/QP7397-Escherichia-coli-strain-K12-Hygromycin-B-4-O-kinase-2-1-300x117.jpghttps://enquirebio.com/wp-content/uploads/2018/01/QP7397-Escherichia-coli-strain-K12-Hygromycin-B-4-O-kinase-3.jpg