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SEQUEST analysis of LC MS/MS spectra obtained from a run with QP8020 identified a match between this protein and the spectra of a peptide sequence that matches a region of Methylosome protein 50.
SEQUEST analysis of LC MS/MS spectra obtained from a run with QP8020 identified a match between this protein and the spectra of a peptide sequence that matches a region of Methylosome protein 50.Additional SEQUEST analysis of the LC MS/MS spectra from QP8020 identified an additional between this protein and the spectra of another peptide sequence that matches a region of Methylosome protein 50 confirming successful recombinant synthesis.SDS-PAGE separation of QP8020 followed by commassie total protein stain results in a primary band consistent with reported data for Methylosome protein 50. These data demonstrate Greater than 90% as determined by SDS-PAGE.

Recombinant Human Methylosome protein 50

$ 398.00 – $ 1,728.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP8020
Species: Human
Available Tags: His-SUMO
Host: E. coli
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 312

Product NumberDescriptionPrice
QP8020-ec-100ug Size: 100 ug, Tag: His-SUMO $ 398.00
QP8020-ec-1mg Size: 1 mg, Tag: His-SUMO $ 1,728.00
Shipping Information
7-10 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
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Concentration (Write Lyophilized if Lyophilized):

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SKU: QP8020-ec-10ug
Recombinant Human Methylosome protein 50 Protein General Information
Alternate Names
p44/Mep50; MEP50; Nbla10071; HKMT1069; MEP-50; p44
Curated Database and Bioinformatic Data
Gene SymbolWDR77
Entrez Gene ID79084
Ensemble Gene IDENSG00000116455
RefSeq Protein Accession(s)NP_001303991.1
RefSeq mRNA Accession(s)NM_001317062.1, NM_001317063.1, NM_001317064.1, NM_024102.3
UniProt ID(s)Q9BQA1
UniGene ID(s)Hs.204773
HGNC ID(s)HGNC:29652
COSMIC ID Link(s)WDR77
KEGG Gene ID(s)hsa:79084
PharmGKB ID(s)PA142670581
General Description of Recombinant Human Methylosome protein 50 Protein.
Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assbly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

Human Methylosome protein 50 Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant Methylosome protein 50 based upon sequence from: Human
Host: QP8020 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Methylosome protein 50, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8020.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Met1 - Leu312
Amino Acid Sequence: MRKETPPPLV PPAAREWNLP PNAPACMERQ LEAARYRSDG ALLLGASSLS GRCWAGSLWL FKDPCAAPNE GFCSAGVQTE AGVADLTWVG ERGILVASDS GAVELWELDE NETLIVSKFC KYEHDDIVST VSVLSSGTQA VSGSKDICIK VWDLAQQVVL SSYRAHAAQV TCVAASPHKD SVFLSCSEDN RILLWDTRCP KPASQIGCSA PGYLPTSLAW HPQQSEVFVF GDENGTVSLV DTKSTSCVLS SAVHSQCVTG LVFSPHSVPF LASLSEDCSL AVLDSSLSEL FRSQAHRDFV RDATWSPLNH SL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Methylosome protein 50 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human Methylosome protein 50

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