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Recombinant Human OTUB1 / OTB1 Protein

$ 148.00$ 1,678.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP8451
Species: Human
Available Tags: GST
Host: E. coli
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 200

Size
Tag Clear selection
Shipping Information
4-6 weeks
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP8451-ec-10ug
Recombinant Human OTUB1 / OTB1 Protein General Information
Alternate Names
OTUB1; OTB1; OTB1; HSPC263; OTU1
Curated Database and Bioinformatic Data
Gene SymbolOTUB1
Entrez Gene ID55611
Ensemble Gene IDENSG00000167770
RefSeq Protein Accession(s)NP_060140.2
RefSeq mRNA Accession(s)NM_017670.2
UniProt ID(s)Q96FW1
UniGene ID(s)Hs.473788
HGNC ID(s)HGNC:23077
COSMIC ID Link(s)OTUB1
KEGG Gene ID(s)hsa:55611
PharmGKB ID(s)PA134988141
General Description of Recombinant Human OTUB1 / OTB1 Protein.
Hydrolase that can specifically rove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of roving NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain.

Human OTUB1 / OTB1 Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant OTUB1 / OTB1 based upon sequence from: Human
Host: QP8451 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) OTUB1 / OTB1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8451.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Ala2 - Lys201
Amino Acid Sequence: AAEEPQQQKQ EPLGSDSEGV NCLAYDEAIM AQQDRIQQEI AVQNPLVSER LELSVLYKEY AEDDNIYQQK IKDLHKKYSY IRKTRPDGNC FYRAFGFSHL EALLDDSKEL QRFKAVSAKS KEDLVSQGFT EFTIEDFHNT FMDLIEQVEK QTSVADLLAS FNDQSTSDYL VVYLRLLTSG YLQRESKFFE HFIEGGRTVK
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human OTUB1 / OTB1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human OTUB1 / OTB1 Protein