SDS-PAGE separation of QP8779 followed by commassie total protein stain results in a primary band consistent with reported data for Vitamin B12-binding protein. These data demonstrate Greater than 90% as determined by SDS-PAGE.

Recombinant E. coli Vitamin B12-binding Protein

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SKU: QP8779
Species: E. coli
Available Tags: GST
Host: E. coli
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 243

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On Demand Protein: 3-5 Week Lead Time. (Why?)
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
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Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

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Purity:

Bioactivity (test results eg. IU/ml):


SKU: QP8779-ec-10ug
Recombinant E. coli Vitamin B12-binding protein Protein General Information
Alternate Names
ECK0157; JW0154; b0158; yadT
Curated Database and Bioinformatic Data
Gene SymbolbtuF
Entrez Gene ID947574
RefSeq Protein Accession(s)NP_414700.1
RefSeq mRNA Accession(s)NC_000913.3, NZ_LN832404.1
UniProt ID(s)P37028
KEGG Gene ID(s)ecj:JW0154
General Description of Recombinant E. coli Vitamin B12-binding protein Protein.
Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC.

E. coli Vitamin B12-binding protein Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant Vitamin B12-binding protein based upon sequence from: E. coli
Host: QP8779 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Vitamin B12-binding protein, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8779.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Pro24 - Asp266
Amino Acid Sequence: PRVITLSPAN TELAFAAGIT PVGVSSYSDY PPQAQKIEQV STWQGMNLER IVALKPDLVI AWRGGNAERQ VDQLASLGIK VMWVDATSIE QIANALRQLA PWSPQPDKAE QAAQSLLDQY AQLKAQYADK PKKRVFLQFG INPPFTSGKE SIQNQVLEVC GGENIFKDSR VPWPQVSREQ VLARSPQAIV ITGGPDQIPK IKQYWGEQLK IPVIPLTSDW FERASPRIIL AAQQLCNALS QVD
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Vitamin B12-binding protein Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant E. coli Vitamin B12-binding Protein