SDS-PAGE separation of QP8853 followed by commassie total protein stain results in a primary band consistent with reported data for Replication protein A 14 kDa subunit. These data demonstrate Greater than 90% as determined by SDS-PAGE.

Recombinant Human Replication protein A 14 kDa subunit Protein

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SKU: QP8853
Species: Human
Available Tags: His
Host: E. coli
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 119

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Product Datasheet
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Bioactivity (test results eg. IU/ml):

SKU: QP8853-ec-10ug
Recombinant Human Replication protein A 14 kDa subunit Protein General Information
Alternate Names
RP-A p14; REPA3
Curated Database and Bioinformatic Data
Gene SymbolRPA3
Entrez Gene ID6119
Ensemble Gene IDENSG00000106399
RefSeq Protein Accession(s)NP_002938.1
RefSeq mRNA Accession(s)NM_002947.4
UniProt ID(s)P35244
UniGene ID(s)Hs.487540
HGNC ID(s)HGNC:10291
KEGG Gene ID(s)hsa:6119
PharmGKB ID(s)PA34653
General Description of Recombinant Human Replication protein A 14 kDa subunit Protein.
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage . In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response . It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair . Plays also a role in base excision repair (BER), probably through interaction with UNG . Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA . As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange .

Human Replication protein A 14 kDa subunit Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant Replication protein A 14 kDa subunit based upon sequence from: Human
Host: QP8853 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Replication protein A 14 kDa subunit, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8853.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Met1 - Gln119
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Replication protein A 14 kDa subunit Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human Replication protein A 14 kDa subunit Protein