SEQUEST analysis of LC MS/MS spectra obtained from a run with QP8929 identified a match between this protein and the spectra of a peptide sequence that matches a region of Ag85A.

Additional SEQUEST analysis of the LC MS/MS spectra from QP8929 identified an additional between this protein and the spectra of another peptide sequence that matches a region of Ag85A confirming successful recombinant synthesis.

Recombinant M. tuberculosis Ag85A Protein

$ 298.00 – $ 1,198.00

Please Select Product Options Below To View The Catalog Number.

SKU: QP8929
Species: M. tuberculosis
Available Tags: His
Host: E. coli
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 279

Product Number	Description	Price
QP8929-ec-10ug	Size: 10 ug, Tag: His	$ 298.00
QP8929-ec-200ug	Size: 200 ug, Tag: His	$ 1,198.00

Shipping Information

On Demand Protein: 3-5 Week Lead Time. (Why?)

Flat Rate Shipping Anywhere in the US: $45

Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: QP8929-ec-10ug

Recombinant M. tuberculosis Ag85A Protein General Information
Alternate Names
; Diacylglycerol acyltransferase/mycolyltransferase Ag8
Curated Database and Bioinformatic Data
Gene Symbol	fbpA
RefSeq Protein Accession(s)	WP_003900759.1
RefSeq mRNA Accession(s)	NZ_KK341227.1
UniProt ID(s)	P9WQP2
KEGG Gene ID(s)	mtc:MT3911
General Description of Recombinant M. tuberculosis Ag85A Protein.
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor (By similarity).

M. tuberculosis Ag85A Recombinant Protein Product Attributes

Product Type: Recombinant Protein

Recombinant Ag85A based upon sequence from: M. tuberculosis

Host: QP8929 protein expressed in E. coli.

Tag: His

Protein Construction: A DNA sequence encoding the Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) Ag85A, was expressed in the hosts and tags indicated. Please select your host/tag option, above.

Application Notes: Please contact us for application specific information for QP8929.

Bioactivity Data: Untested

Full Length? Partial (see sequence information for more details).

Expression Region: Tyr53 - Thr331

Amino Acid Sequence: YLQVPSPSMG RDIKVQFQSG GANSPALYLL DGLRAQDDFS GWDINTPAFE WYDQSGLSVV MPVGGQSSFY SDWYQPACGK AGCQTYKWET FLTSELPGWL QANRHVKPTG SAVVGLSMAA SSALTLAIYH PQQFVYAGAM SGLLDPSQAM GPTLIGLAMG DAGGYKASDM WGPKEDPAWQ RNDPLLNVGK LIANNTRVWV YCGNGKPSDL GGNNLPAKFL EGFVRTSNIK FQDAYNAGGG HNGVFDFPDS GTHSWEYWGA QLNAMKPDLQ RALGATPNT

Purity: Greater than 90% as determined by SDS-PAGE.

Reconstitution Instructions:

Concentration of M. tuberculosis Ag85A Protein:

Endotoxin Levels: Not determined.

Buffer: Tris-based buffer, 50% glycerol

Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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