SDS-PAGE separation of QP9447 followed by commassie total protein stain results in a primary band consistent with reported data for E3 ubiquitin-protein ligase TRIM21. These data demonstrate Greater than 90% as determined by SDS-PAGE.

Recombinant Human E3 ubiquitin-protein ligase TRIM21 Protein

$ 528.00$ 1,458.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP9447
Species: Human
Available Tags: His
Host: Yeast
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 475

Product NumberDescriptionPrice
QP9447-ye-100ug Size: 100 ug, Tag: His $ 528.00
QP9447-ye-500ug Size: 500 ug, Tag: His $ 1,458.00
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On Demand Protein: 3-5 Week Lead Time. (Why?)
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

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Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

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Purity:

Bioactivity (test results eg. IU/ml):


SKU: QP9447-ye-10ug
Recombinant Human E3 ubiquitin-protein ligase TRIM21 Protein General Information
Alternate Names
RNF81; Ro/SSA; RO52; SSA; SSA1
Curated Database and Bioinformatic Data
Gene SymbolTRIM21
Entrez Gene ID6737
Ensemble Gene IDENSG00000132109
RefSeq Protein Accession(s)NP_003132.2
RefSeq mRNA Accession(s)NM_003141.3
UniProt ID(s)P19474
UniGene ID(s)Hs.532357
HGNC ID(s)HGNC:11312
COSMIC ID Link(s)TRIM21
KEGG Gene ID(s)hsa:6737
PharmGKB ID(s)PA36136
General Description of Recombinant Human E3 ubiquitin-protein ligase TRIM21 Protein.
E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2

Human E3 ubiquitin-protein ligase TRIM21 Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant E3 ubiquitin-protein ligase TRIM21 based upon sequence from: Human
Host: QP9447 protein expressed in Yeast.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) E3 ubiquitin-protein ligase TRIM21, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP9447.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 - Tyr475
Amino Acid Sequence: MASAARLTMM WEEVTCPICL DPFVEPVSIE CGHSFCQECI SQVGKGGGSV CPVCRQRFLL KNLRPNRQLA NMVNNLKEIS QEAREGTQGE RCAVHGERLH LFCEKDGKAL CWVCAQSRKH RDHAMVPLEE AAQEYQEKLQ VALGELRRKQ ELAEKLEVEI AIKRADWKKT VETQKSRIHA EFVQQKNFLV EEEQRQLQEL EKDEREQLRI LGEKEAKLAQ QSQALQELIS ELDRRCHSSA LELLQEVIIV LERSESWNLK DLDITSPELR SVCHVPGLKK MLRTCAVHIT LDPDTANPWL ILSEDRRQVR LGDTQQSIPG NEERFDSYPM VLGAQHFHSG KHYWEVDVTG KEAWDLGVCR DSVRRKGHFL LSSKSGFWTI WLWNKQKYEA GTYPQTPLHL QVPPCQVGIF LDYEAGMVSF YNITDHGSLI YSFSECAFTG PLRPFFSPGF NDGGKNTAPL TLCPLNIGSQ GSTDY
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human E3 ubiquitin-protein ligase TRIM21 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human E3 ubiquitin-protein ligase TRIM21 Protein