SDS-PAGE separation of QP7725 followed by commassie total protein stain results in a primary band consistent with reported data for E3 ubiquitin-protein ligase TRIM21. These data demonstrate Greater than 90% as determined by SDS-PAGE.

Recombinant Mouse E3 ubiquitin-protein ligase TRIM21 Protein

$ 228.00 – $ 2,468.00

Please Select Product Options Below To View The Catalog Number.

SKU: QP7725
Species: Mouse
Available Tags: His-SUMO
Host: E. coli
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 470

Product Number	Description	Price
QP7725-ec-10ug	Size: 10 ug, Tag: His-SUMO	$ 228.00
QP7725-ec-100ug	Size: 100 ug, Tag: His-SUMO	$ 578.00
QP7725-ec-1mg	Size: 1 mg, Tag: His-SUMO	$ 2,468.00

Shipping Information

On Demand Protein: 3-5 Week Lead Time. (Why?)

Flat Rate Shipping Anywhere in the US: $45

Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: QP7725-ec-10ug

Recombinant Mouse E3 ubiquitin-protein ligase TRIM21 Protein General Information
Alternate Names
Ro52; Ssa1
Curated Database and Bioinformatic Data
Gene Symbol	TRIM21
UniProt ID(s)	Q62191
UniGene ID(s)	Mm.321227
COSMIC ID Link(s)	TRIM21
General Description of Recombinant Mouse E3 ubiquitin-protein ligase TRIM21 Protein.
E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2.

Mouse E3 ubiquitin-protein ligase TRIM21 Recombinant Protein Product Attributes

Product Type: Recombinant Protein

Recombinant E3 ubiquitin-protein ligase TRIM21 based upon sequence from: Mouse

Host: QP7725 protein expressed in E. coli.

Tag: His-SUMO

Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) E3 ubiquitin-protein ligase TRIM21, was expressed in the hosts and tags indicated. Please select your host/tag option, above.

Application Notes: Please contact us for application specific information for QP7725.

Bioactivity Data: Untested

Full Length? Full Length

Expression Region: Met1 - Met470

Amino Acid Sequence: MSPSTTSKMS LEKMWEEVTC SICLDPMVEP MSIECGHCFC KECIFEVGKN GGSSCPECRQ QFLLRNLRPN RHIANMVENL KQIAQNTKKS TQETHCMKHG EKLHLFCEED GQALCWVCAQ SGKHRDHTRV PIEEAAKVYQ EKIHVVLEKL RKGKELAEKM EMDLTMQRTD WKRNIDTQKS RIHAEFALQN SLLAQEEQRQ LQRLEKDQRE YLRLLGKKEA ELAEKNQALQ ELISELERRI RGSELELLQE VRIILERSGS WNLDTLDIDA PDLTSTCPVP GRKKMLRTCW VHITLDRNTA NSWLIISKDR RQVRMGDTHQ NVSDNKERFS NYPMVLGAQR FSSGKMYWEV DVTQKEAWDL GVCRDSVQRK GQFSLSPENG FWTIWLWQDS YEAGTSPQTT LHIQVPPCQI GIFVDYEAGV VSFYNITDHG SLIYTFSECV FAGPLRPFFN VGFNYSGGNA APLKLCPLKM

Purity: Greater than 90% as determined by SDS-PAGE.

Reconstitution Instructions:

Concentration of Mouse E3 ubiquitin-protein ligase TRIM21 Protein:

Endotoxin Levels: Not determined.

Buffer: Tris-based buffer, 50% glycerol

Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

There are no reviews yet.