PDF DatasheetHuman LAGE3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant LAGE3 based upon sequence from Human
Host: QP12524 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of LAGE3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12524.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMRDADAD AGGGADGGDG RGGHSCRGGV DTAAAPAGGA PPAHAPGPGR DAASAARGSR MRPHIFTLSV PFPTPLEAEI AHGSLAPDAE PHQRVVGKDL TVSGRILVVR WKAEDCRLLR ISVINFLDQL SLVVRTMQRF GPPVSR
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The LAGE3 solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 50% glycerol and 2mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human LAGE3 General Information | |
|---|---|
| Alternate Names | |
| CVG5, DXS9879E, DXS9951E, ESO3, ITBA2, EKC/KEOPS complex subunit LAGE3, L antigen family member 3, Protein ESO-3. | |
| Molecular Weight | |
| 17.2 kDa | |
| Chromosomal Location | |
| q28 on chromosome X | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | LAGE3 |
| Entrez Gene ID | 8270 |
| UniProt ID(s) | Q14657 |
| COSMIC ID Link(s) | LAGE3 |
| KEGG Gene ID(s) | hsa:8270 |
| General Description of Recombinant Human LAGE3. | |
| Human LAGE3 produced in E. coli is a single polypeptide chain containing 166 amino acids (1-143) and having a molecular mass of 17.2kDa. LAGE3 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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