PDF DatasheetHuman ALKBH3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ALKBH3 based upon sequence from: Human
Isoform: Isoform 2
Host: QP7948 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) ALKBH3, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7948.
Bioactivity Data: Untested
Full Length? Full Length of Isoform 2
Expression Region: Met1 – Pro170
Amino Acid Sequence: MEEKRRRARV QGAWAAPVKS QAIAQPATTA KSHLHQKPGQ TWKNKEHHLS DREFVFKEPQ QVVRRAPEPR VIEEGVYEIS LSPTGVSRVC LYPGFVDVKE ADWILEQLCQ DVPWKQRTGI REDSILQLTF KKSAPVSGTA TAPQSCWYER PSPPHIPGPA ILTRTRLWAP
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human ALKBH3 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human ALKBH3 Protein General Information | |
|---|---|
| Alternate Names | |
| DEPC1; hABH3; ABH3; DEPC-1; PCA1; Alpha-ketoglutarate-dependent dioxygenase alkB homolog | |
| Molecular Weight | |
| Isoform 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ALKBH3 |
| Entrez Gene ID | 221120 |
| Ensemble Gene ID | ENSG00000166199 |
| RefSeq Protein Accession(s) | NP_631917.1 |
| RefSeq mRNA Accession(s) | NM_139178.3 |
| UniProt ID(s) | Q96Q83 |
| UniGene ID(s) | Hs.720708 |
| HGNC ID(s) | HGNC:30141 |
| COSMIC ID Link(s) | ALKBH3 |
| KEGG Gene ID(s) | hsa:221120 |
| PharmGKB ID(s) | PA143485293 |
| General Description of Recombinant Human ALKBH3 Protein. | |
| Dioxygenase that repairs alkylated DNA containing 1-methyladenine (1meA) and 3-methylcytosine (3meC) by oxidative dethylation. Has a strong preference for single-stranded DNA. Able to process alkylated 3mC within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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