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QP15354, CCR4 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CCR4 based upon sequence from Homo sapiens (Human)
Host: QP15354 protein expressed in in vitro E.coli expression system.
Available Tags: N-terminal 10xHis-tagged
Protein Construction: A cDNA sequence encoding the sequence of CCR4 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Western Blot, ELISA and others
Application Notes: Please contact us for application specific information for QP15354.
Bioactivity Data: ①Measured by its binding ability in a functional ELISA. Immobilized Human CCR4 at 5 ug/mL can bind Anti-CCR4 recombinant antibody (CSB-RA004843MA01HU). The EC50 is 2.803-3.369 ng/mL.
②Human CCR4 Full Length Protein captured on CM5 chip can bind Human CCR4 Monoclonal Antibody (CSB-RA004843MA01HU) with an affinity constant of 3.16 nM as detected by SPR Assay(Biacore T200).
③Human CCR4 Monoclonal Antibody (CSB-RA004843MA01HU) captured on Protein A Chip can bind Human CCR4 Full Length Protein with an affinity constant of 1.71 nM as detected by MetaSPR Assay (in presence of FOS-12)(WeSPRTM200) .
②Human CCR4 Full Length Protein captured on CM5 chip can bind Human CCR4 Monoclonal Antibody (CSB-RA004843MA01HU) with an affinity constant of 3.16 nM as detected by SPR Assay(Biacore T200).
③Human CCR4 Monoclonal Antibody (CSB-RA004843MA01HU) captured on Protein A Chip can bind Human CCR4 Full Length Protein with an affinity constant of 1.71 nM as detected by MetaSPR Assay (in presence of FOS-12)(WeSPRTM200) .
Amino Acid Sequence: MNPTDIADTTLDESIYSNYYLYESIPKPCTKEGIKAFGELFLPPLYSLVFVFGLLGNSVVVLVLFKYKRLRSMTDVYLLNLAISDLLFVFSLPFWGYYAADQWVFGLGLCKMISWMYLVGFYSGIFFVMLMSIDRYLAIVHAVFSLRARTLTYGVITSLATWSVAVFASLPGFLFSTCYTERNHTYCKTKYSLNSTTWKVLSSLEINILGLVIPLGIMLFCYSMIIRTLQHCKNEKKNKAVKMIFAVVVLFLGFWTPYNIVLFLETLVELEVLQDCTFERYLDYAIQATETLAFVHCCLNPIIYFFLGEKFRKYILQLFKTCRGLFVLCQYCGLLQIYSADTPSSSYTQSTMDHDLHDAL
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: See COA, sent with protein.
Endotoxin Levels: Not test
Buffer: Lyophilized from PBS, 0.05% FOS-12, 6% Trehalose, pH 8.0.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: White powder.
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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