PDF DatasheetAT1:AU2
QP15346, CD47 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CD47 based upon sequence from Homo sapiens (Human)
Host: QP15346 protein expressed in Mammalian cell.
Available Tags: C-terminal 10xHis-tagged
Protein Construction: A cDNA sequence encoding the sequence of CD47 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Western Blot, ELISA and others
Application Notes: Please contact us for application specific information for QP15346.
Bioactivity Data: ①Measured by its binding ability in a functional ELISA. Immobilized Human CD47 (CSB-MP004940HUd7) at 2 ug/mL can bind Human SIRPA protein. The EC50 is 98.73-112.7 ng/mL.
②Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 2 ug/mL can bind Anti-CD47 recombinant antibody (CSB-RA004940MA1HU). The EC50 is 1.343-1.561 ng/mL.
②Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 2 ug/mL can bind Anti-CD47 recombinant antibody (CSB-RA004940MA1HU). The EC50 is 1.343-1.561 ng/mL.
Amino Acid Sequence: QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSP
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: See COA, sent with protein.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: White powder.
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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