Western blot data demonstrating successful knockdown of ApoE by QX5 at both 24 and 48 hrs post transfection (TC = Transfection Control, SC = Scrambled Control (Product Number QC1), siRNA = QX5 treatment)
Western blot data demonstrating successful knockdown of ApoE by QX5 at both 24 and 48 hrs post transfection (TC = Transfection Control, SC = Scrambled Control (Product Number QC1), siRNA = QX5 treatment)

TranslationBlocker Human ApoE siRNA

$ 219.00$ 329.00
Please Select Product Options Below To View The Catalog Number.

Human ApoE siRNA Translation Blocker™ Duplex

Product NumberDescriptionPrice
QX5-2nmol Size: 2 nmol, Tag: Untagged (Native Protein) $ 248.00
QX5-10nmol Size: 10 nmol, Tag: Untagged (Native Protein) $ 368.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QX5-2nmol

Human ApoE TranslationBlocker™ siRNA Duplex Product Attributes

Blocks transcription and translation of ApoE from: Human.
May also react with ApoE based upon sequence homology and clone specific publications.
Type of RNAi: siRNA.
siRNA target sequence: NM_000041.
Samples and cell lines tested: Ovcar3 Human Ovarian Cancer Cells.
Buffer and Stabilizer: Lyophilized. See datasheet for resuspension instructions and buffer information.
Concentration of Anti-Human ApoE: Lyophilized
SiRNA Purification Method:RPC/HPLC
Storage Conditions: Store Lyophilized siRNA duplex at 4C. Reconsituted product may be stored at -20C for up to 1 month.
    ApoE Knockdown Protocol:
    1. Starting with cultured cells in growth media seed 24 well plates with 5 x 104 cells and incubate at 37°C for 24 hours.
    2. Prepare transfection reagent in serum-free growth media allow to come to room temperature. The above data was generated with Oligofectamine prepared in accordance with manufacturer’s instructions.
    3. Dilute siRNA to working concentration in serum-free growth media. Optimal dilution should be experimentally determined.
    4. Combine diluted siRNA and transfection reagent. Equivalent volumes of prepared Oligofectamine and diluted siRNA should be combined and incubated at room temperature for 20 minutes with gentle mixing (optional).
    5. Add prepared siRNA solution to cells.
    6. Incubate for several hours in serum free media.
    7. Add serum containing media to culture without a media change. Generally 1-2 fold of the original volume of siRNA containing serum free media is added to restore nutrient levels in media.
    8. Incubate and assay several hours after transfection. An additional transfection may be performed, as above, 24 hours after initial transfection to enhance knockdown. Optimal knockdown time should also be experimentally determined. In the above experimental conditions, Apolipoprotein E (ApoE) knockdown at the protein level was not visible until 24 hours post transfection. Knockdown continued to improve

ApoE General Information

Gene Name: APOE
Entrez Gene ID: 348
NCBI Ref Seq Accession Number: NP_000032
Uniprot ID:
ApoE Molecular Weight:
Alternate Names: AD2, APO-E, LPG, LDLCQ5, ApoE4, ApoE, Apolipoprotein E

Limitations and Warranty

This product is for Research Use Only. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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TranslationBlocker Human ApoE siRNA

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