IHC / Immunohistochemistry Protocol
One Final Tip: Use Highly Validated Antibodies!
One Final Tip: Use Highly Validated Antibodies!
What Sets Our IHC Antibodies Apart Our monoclonal antibodies for immunohistochemistry (IHC) are sourced from the best in the business: NeoBiotechnologies. Although you may not be familiar with NeoBiotechnologies, we will outline what sets them apart from other companies and why we chose them as a partner for our IHC monoclonal antibodies. We believe you will...
Our Mission: Better, truly quantitative antibody validation. Click here for details on our Mass Spec MS-QAVA™ Validation Methods and to see our MS-QAVA™ mAbs. MS-QAVA™: Mass Spec Based Quantitative Antibody Validation. Our products will take the guess work out of determining antibody specificity with a novel Mass-Spec based validation method. Click to learn more. Even CRISPR validation only...
MS QAVA™: Quantitative Measurement of Antibody Specificity Powered by Mass Spec. Existing validation only provides approximations and assumptions of specificity. Here’s how it works: Immunoprecipitation and Mass Spec is run for every fraction for a total of 36-72 sample types per antibody. Specificity is calculated as antibody vs. mass spec r-squared. r-squared represents variability in...
Anti-Human Thyroglobulin mAbs. We carry the top published clones for IHC. There are numerous Thyroglobulin (TGB) monoclonal antibodies that have been used in publications. 2H11, 6E1, SPM221 and SOM517, and TGB04, TGB05, and TGB24 produced acceptable staining patterns. A cocktail of 2H11 and 6E1 is also available to aid in optimization of staining as these clones...
PD1 Antibodies and Recombinant Proteins. We carry the top published PD1 clones for IHC. There are numerous Programmed Cell Death Protein 1 (PD1, PDCD1, CD279) monoclonal antibodies that have been used in publications. PDCD1/922 and SPM597 performed the best in immunohistochemical (IHC) staining. Our newest recombinant monoclonal PDCD1/1410R is a great IHC antibody with the reproducibility...
Anti-BrdU Monoclonal Antibodies for IHC We carry the top published clones for IHC. There are numerous Anti Bromodoxyuridine (BrdU) monoclonal antibodies that have been used in publications. BrdU is a thymadine analog that is added to proliferating cells. It is incorporated into DNA of dividing cells and an Anti-BrdU antibody can then be used to...
Anti-CD63 Monoclonal Antibodies and CD63 Recombinant Proteins. Identical anti-CD63 clones are not the same antibodies! All of our anti-human and anti-mouse CD63 antibodies are produced in bioreactors to reduce the amount of non-immune Ig present in our products. Non-immune Ig is derived from ascites or serum in traditional hybridoma culture. It may represent as much...
Anti-Actin (Smooth Muscle Specific) Monoclonal Antibodies We carry the top published Anti-Actin clones for IHC. There are numerous anti-actin monoclonal antibodies available. Clone 1A4, SPM332, and ACTA2/791 are specific to actin in smooth muscle. The first two images show specific staining of smooth muscle in Leiomyosarcoma. The second is staining in angiosarcoma. Staining was preceeded...
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