Anti-CD14 Antibody [61D3]

$ 88.00$ 968.00

Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;APC ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

SKU: QAB26-F-25Tests Categories: , , , , , , Tag:
 PDF Datasheet

Human Anti-CD14 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 µl per test where one test represents staining of a cell sample in a final volume of approximately 100 µL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD14 Antibody Clone: 61D3
Clone 61D3 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2
Antibody Concentration: 5 &microL/test
Storage Conditions: 2-8°C protected from light. Stable for 12 Months. Do Not Freeze Conjugated Formats.

CD14 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD14 antibody reveals CD14 expression is expected to be primarily localized to the plasma membrane and vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in CD14 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the colon. More moderate antibody staining intensity was present in endothelial cells in the colon. Low, but measureable presence of CD14 could be seen in cells in the seminiferous ducts in testis, fibroblasts in skin, glandular cells in the fallopian tube and salivary gland, Langerhans in skin and Leydig cells in the testis. We were unable to detect CD14 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD14 expression as measured by anti-CD14 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity
CD14 Variability + ++ + + + + + ++ + + + ++ ++ + + + + + + +

Selected References

Hogg N, Horton MA. 1987. In: McMichael AJ, Beverley PCL, Cobbold S, et al., eds. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 576-602. Haziot A, Chen S, Ferrero E, Low MG, Silber R, Goyert SM. 1988. J Immunol. 141:547-552.

Limitations and Warranty

enQuire Bio’s Human Anti-CD14 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Buffer and Stabilizer

Product Type





Mass Spec Validated?

  1. Kang et al.

    Flow Cytometry with anti-CD14 (61D3) of human cells from gastric cancer samples.


    • Anti-CD14 (Clone 61D3)
      • Use conjugated primary antibodies (e.g. FITC, PE, APC)
    • Mouse IgG1 Isotype Control (conjugated to the same fluorophore as 61D3).
    • Single Cell Suspension from Gastric Cancer Patient Tumor Samples
    • PBS
    • 40 μm nylon filter, 15 ml conical tubes, scissors, 3 ml syringe plunger
    • Flow cytometry staining tubes
    • Pipettes, tips, other consumables
    • FACS Canto II Flow Cytometer


    1. Prepare cell suspension from tumor samples.
      1. Using 3mm syringe plunger, mechanically disrupt tissue and press through nylon filter in the presence of PBS.
      2. Wash and resuspend cells in 1ml PBS (add 1ml of PBS, pellet cells at 400 x rcf, aspirate supernatant, and resuspend in 1ml PBS).
      3. Count cells using a hemocytometer or automated cell counter.
      4. Aliquot 1 x 106 cells to separate tubes.
    2. Stain Cells with Anti-CD14 and isotype control.
      1. Wash and resuspend cells as above.
      2. Add 5 ul of Anti-CD14 to each tube containing 1×106 cells. Specific dilutions here were used in an 11 color panel to distinguish tumor infiltrating lymphocytes, t-cell subsets, NK cells, and other tumor cells and should be confirmed,especially with regard to different fluorophore conjugates.
      3. Incubate samples on ice in the dark for 30 min.
      4. Wash and resuspend cells 2x as described above.
      5. Other extracellular targets may be labeled at the same time as CD14.
      6. If intracellular targets are to be stained, proceed to intracellular staining after surface marker staining is complete.
    3. Analyze Cells
      1. Cells were analyzed on a FACS Canto II Flow Cytometer.
      2. Isotype controls were run for each sample for gating and sample analysis.
      3. Compensation beads matched to the antibody (same fluorophore) were also run to allow proper measurement of each marker (this is only relevant for multicolor analyses).
      4. Additional analysis details can be viewed by visiting the publication.

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Anti-CD14 Antibody [61D3]