PDF DatasheetAntibody DISCONTINUED
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:50 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at RoomTemperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-CD25 Antibody Clone: EP218
Host and Isotype: Rabbit IgG
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody EP218 Staining: Cell Membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to residues of human CD25 protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
CD25 Information for Pathologists
| CD25 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 30.8 kDa | |
| Chromosomal Location | |
| p15.1 [chr: 10] [chr_start: 6010689] [chr_end: 6062370] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IL2RA |
| Entrez Gene ID | 3559 |
| RefSeq Protein Accession(s) | NP_000408; NP_001295171; NP_001295172 |
| RefSeq mRNA Accession(s) | NM_000417; NM_001308242; NM_001308243 |
| RefSeq Genomic Accession(s) | NC_000010; NG_007403 |
| UniProt ID(s) | P01589 |
| PharmGKB ID(s) | PA29828 |
| KEGG Gene ID(s) | hsa:3559 |
| Associated Diseases (KEGG IDs) | Diabetes mellitus, insulin-dependent, 10 (IDDM10) [MIM:601942]: A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical features are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. {ECO:0000269|PubMed:17676041}. Disease susceptibility is associated with variations affecting the gene represented in this entry.; Immunodeficiency 41 with lymphoproliferation and autoimmunity (IMD41) [MIM:606367]: A disorder of immune dysregulation characterized by recurrent viral, fungal, and bacterial infections, lymphadenopathy, and variable autoimmune features, such as autoimmune enteropathy and eczematous skin lesions. {ECO:0000269|PubMed:23416241, ECO:0000269|PubMed:24116927}. The disease is caused by mutations affecting the gene represented in this entry. |
| General Description of CD25 . | |
| CD25, or interleukin 2 (IL2) receptor alpha (IL2RA), and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells and activated B cells. Normally an integral-membrane protein, soluble CD25 has been isolated and determined to result from extracellular proteolysis. CD25 also appears to be a reliable immunohistochemical marker for the discrimination of neoplastic from normal/reactive mast cells, with potential as a diagnostic tool in systemic mastocytosis. CD25 is also expressed on hairy cell leukemia | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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