PDF DatasheetMouse Anti-CD28 Antibody Product Attributes
Species: Mouse
Tested Applications: Flow Cytometry, immunoprecipitation.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD28 Antibody Clone: 37.51
Clone 37.51 Host and Isotype: Golden Syrian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD28 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD28 / TP44 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD28 / TP44 could be seen in cells in the red pulp in spleen, germinal center cells in the tonsil, hematopoietic cells in the bone marrow and lymphoid tissue in appendix. We were unable to detect CD28 / TP44 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| CD28 General Information | |
|---|---|
| Alternate Names | |
| CD28, TP44, CD28RNA | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Cd28 |
| Entrez Gene ID | 12487 |
| Ensemble Gene ID | ENSMUSG00000102412, ENSMUSG00000026012 |
| RefSeq Protein Accession(s) | NP_031668 |
| RefSeq mRNA Accession(s) | NM_007642 |
| RefSeq Genomic Accession(s) | NG_187019, NC_000067, NW_006763128 |
| UniProt ID(s) | P31041, Q8CDB3 |
| UniGene ID(s) | P31041, Q8CDB3 |
| Cosmic ID(s) | Cd28 |
| KEGG Gene ID(s) | mmu:12487 |
| General Description of CD28. | |
| The 37.51 antibody reacts with mouse CD28, a 45 kDa glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of co-stimulatory receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA.The 37.51 may be used as a phenotypic marker for CD28, which is expressed on all CD4+ T cells and CD8+ T cells, and on NK cells in mouse. In addition, the 37.51 antibody is widely used to activate the CD28 receptor in vitro and in vivo. Please choose the appropriate format for each application. | |
Selected References
Johnston RJ, Choi YS, Diamond JA, Yang JA, and Crotty S. 2012. J. Exp. Med. 209:243-250. (in vitro activation)Hafalla JCR, Burgold J, Dorhoi A, Gross O, Ruland J, Kaufmann SHE, and Matuschewski K. 2012. Infect. Immun. 80:1274-1279. (in vitro activation)Driessens G, Zheng Y, Locke F, Cannon JL, Gounari F, and Gajewski TF. 2011. J. Immunol. 186:784-790. (Flow cytometry)Alcazar I, Cortes I, Zaballos A, Hernandez C, Fruman DA, Barber DF, and Carrera AC. 2009. Blood. 113:3198-3208. (Immunoprecipitation, in vitro activationAlbert MH, Yu X-Z, Martin PJ, and Anasetti C. 2005. Blood. 105:1355-1361. (in vivo activation)
Limitations and Warranty
enQuire Bio’s Mouse Anti-CD28 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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