PDF DatasheetHuman Anti-CD54 / ICAM-1 Antibody Product Attributes
CD54 / ICAM-1 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD54 / ICAM-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney, endothelial cells in the colon and pneumocytes in lung. More moderate antibody staining intensity was present in cells in the glomeruli in kidney, endothelial cells in the colon and pneumocytes in lung. Low, but measureable presence of CD54 / ICAM-1 could be seen in cells in the seminiferous ducts in testis, germinal center cells in the lymph node, glandular cells in the fallopian tube and urothelial cells in the urinary bladder. We were unable to detect CD54 / ICAM-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of CD54 / ICAM-1 expression as measured by anti-CD54 / ICAM-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | – | – | – | – | – | – | + | – | – | – | – | + | – | – | – | – | – | – | – | – |
| ICAM1 Variability | ++ | + | ++ | + | + | + | ++ | ++ | ++ | + | ++ | ++ | + | + | ++ | ++ | + | + | + | + |
| CD54 / ICAM-1 General Information | |
|---|---|
| Alternate Names | |
| ICAM1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ICAM1 |
| Entrez Gene ID | 3383 |
| Ensemble Gene ID | ENSG00000090339 |
| RefSeq Protein Accession(s) | NP_000192 |
| RefSeq mRNA Accession(s) | NM_000201 |
| RefSeq Genomic Accession(s) | NC_000019, NG_012083, NC_018930 |
| UniProt ID(s) | P05362 |
| UniGene ID(s) | P05362 |
| HGNC ID(s) | 5344 |
| Cosmic ID(s) | ICAM1 |
| KEGG Gene ID(s) | hsa:3383 |
| PharmGKB ID(s) | PA29592 |
| General Description of CD54 / ICAM-1. | |
| The 15.2 antibody reacts with human CD54, also known as ICAM-1 (Intercellular Adhesion Molecule 1), a 90-110 kDa cell surface glycoprotein that is inducibly expressed on both immune and endothelial cells. As its name implies, ICAM-1 participates in cell-cell adhesion between leukocytes and endothelial cells, facilitating leukocyte recruitment and transmigration at sites of inflammation. The ligands for ICAM-1 are also expressed on leukocyte and endothelial cells, and include Mac-1, fibrinogen, and a member of the integrin protein family, LFA-1 (CD11a).The 15.2 antibody may be used for analysis of ICAM-1 expression in human cells and tissues, and is reported to be cross-reactive with porcine ICAM-1. | |
Selected References
Limitations and Warranty
| Size | |
|---|---|
| Tag | APC, FITC, PE, Qfluor™ 630, Unconjugated |
| Buffer and Stabilizer | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2, 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2 |
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| Mass Spec Validated? |
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