PDF DatasheetHuman, Rat (-), and Mouse (-) Anti-Epcam / CD326 Antibody Product Attributes
Species: Human, Rat (-), and Mouse (-)
Tested Applications: Immunohistochemistry (IHC).
Clonality: Recombinant Monoclonal
Anti-Epcam / CD326 Antibody Clone: EGP40/1555R
Clone EGP40/1555R Host and Isotype: Rabbit IgG kappa
Anti-Human, Rat (-), and Mouse (-) Epcam / CD326 Positive Control Sample: HT29 cells or breast tumor
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with or without 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide (around aa 20-60) from the N-terminus of human EPCAM protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Epcam / CD326 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-EPCAM antibody staining is expected to be primarily localized to the plasma membrane.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Epcam / CD326 expression as measured by anti-Epcam / CD326 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | – | + | + | +++ | +++ | – | – | – | +++ | – | – | +++ | – | +++ | – | – | – | – | +++ | – |
| EPCAM Variability | ++ | +++ | +++ | + | + | + | ++ | + | ++ | + | + | ++ | + | + | + | ++ | ++ | ++ | + | + |
| Epcam / CD326 General Information | |
|---|---|
| Alternate Names | |
| EpCAM, Epithelial cell adhesion molecule, EGP40, CD326, Cluster of Differentiation 326 | |
| Molecular Weight | |
| 40-43kDa | |
| Chromosomal Location | |
| 2q21 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | EPCAM |
| Entrez Gene ID | 4072 |
| Ensemble Gene ID | ENSG00000119888 |
| RefSeq Protein Accession(s) | NP_002345 |
| RefSeq mRNA Accession(s) | NM_002354, |
| RefSeq Genomic Accession(s) | NG_012352, NC_000002, NC_018913 |
| UniProt ID(s) | P16422 |
| UniGene ID(s) | P16422 |
| HGNC ID(s) | 11529 |
| Cosmic ID(s) | EPCAM |
| KEGG Gene ID(s) | hsa:4072 |
| PharmGKB ID(s) | PA35493 |
| General Description of Epcam / CD326. | |
| Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Epcam). Epcam is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin. | |
Limitations and Warranty
enQuire Bio’s Epcam / CD326 Recombinant Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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