PDF DatasheetHuman, Rat (-), and Ferret (-) Anti-Epcam / CD326 Antibody Product Attributes
Species: Human, Rat (-), and Ferret (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Epcam / CD326 Antibody Clone: VU-1D9
Clone VU-1D9 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Rat (-), and Ferret (-) Epcam / CD326 Positive Control Sample: HT29 cells or breast tumor
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Small cell lung carcinoma cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Epcam / CD326 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-EPCAM antibody staining is expected to be primarily localized to the plasma membrane.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Epcam / CD326 expression as measured by anti-Epcam / CD326 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | – | + | + | +++ | +++ | – | – | – | +++ | – | – | +++ | – | +++ | – | – | – | – | +++ | – |
| EPCAM Variability | ++ | +++ | +++ | + | + | + | ++ | + | ++ | + | + | ++ | + | + | + | ++ | ++ | ++ | + | + |
| Epcam / CD326 General Information | |
|---|---|
| Alternate Names | |
| EpCAM, Epithelial cell adhesion molecule, EGP40, CD326, Cluster of Differentiation 326 | |
| Molecular Weight | |
| 40-43kDa | |
| Chromosomal Location | |
| 2p21 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | EPCAM |
| Entrez Gene ID | 4072 |
| Ensemble Gene ID | ENSG00000119888 |
| RefSeq Protein Accession(s) | NP_002345 |
| RefSeq mRNA Accession(s) | NM_002354, |
| RefSeq Genomic Accession(s) | NG_012352, NC_000002, NC_018913 |
| UniProt ID(s) | P16422 |
| UniGene ID(s) | P16422 |
| HGNC ID(s) | 11529 |
| Cosmic ID(s) | EPCAM |
| KEGG Gene ID(s) | hsa:4072 |
| PharmGKB ID(s) | PA35493 |
| General Description of Epcam / CD326. | |
| This antibody reacts with the first EGF repeat in the extracellular domain of Epcam. It is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Epcam). It is expressed on baso-lateral cell surface in most simple epithelia, a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes, gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma, hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. | |
Limitations and Warranty
enQuire Bio’s Epcam / CD326 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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