PDF DatasheetHuman, Canine (-), and Rat (-) Anti-gp100 / Melanosome / PMEL17 / SILV Antibody Product Attributes
Species: Human, Canine (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-gp100 / Melanosome / PMEL17 / SILV Antibody Clone: HMB45
Clone HMB45 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Canine (-), and Rat (-) gp100 / Melanosome / PMEL17 / SILV Positive Control Sample: SK-MEL-28 cells or Melanoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Extract of pigmented melanoma metastases from lymph nodes
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
gp100 / Melanosome / PMEL17 / SILV Previously Observed Antibody Staining Patterns
| gp100 / Melanosome / PMEL17 / SILV General Information | |
|---|---|
| Alternate Names | |
| Melanocyte protein PMEL, Premelanosome protein, PMEL, Silver locus protein homolog, SILV | |
| Molecular Weight | |
| 90-100kDa | |
| Chromosomal Location | |
| 12q13.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SILV |
| Entrez Gene ID | 6490 |
| Ensemble Gene ID | ENSG00000185664 |
| RefSeq Protein Accession(s) | NP_001186982, XP_006719632, NP_001307050, NP_008859, XP_011536987, NP_001186983, NP_001307051 |
| RefSeq mRNA Accession(s) | XM_006719569, NM_001320122, NM_001200053, XM_011538685, NM_001320121, NM_006928 NM_001200054 |
| RefSeq Genomic Accession(s) | NC_000012, NC_018923, NG_028086 |
| UniProt ID(s) | P40967 |
| UniGene ID(s) | P40967 |
| HGNC ID(s) | 10880 |
| Cosmic ID(s) | PMEL |
| KEGG Gene ID(s) | hsa:6490 |
| PharmGKB ID(s) | PA35781 |
| General Description of gp100 / Melanosome / PMEL17 / SILV. | |
| By immunohistochemistry, it specifically recognizes a protein in melanocytes, melanomas. This MAb reacts with junctional, blue nevus cells, variably with fetal, neonatal melanocytes. Intradermal nevi, normal adult melanocytes,, non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas,, sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas, various types of mesenchymal neoplasms. This MAb stains fetal, neonatal melanocytes, junctional, blue nevus cells,, malignant melanoma. This MAb also stains Angiomyolipoma (PEComa). | |
Limitations and Warranty
enQuire Bio’s gp100 / Melanosome / PMEL17 / SILV Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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