PDF DatasheetHuman Anti-gp100 / Melanosome / PMEL17 / SILV Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-gp100 / Melanosome / PMEL17 / SILV Antibody Clone: PMEL/783
Clone PMEL/783 Host and Isotype: Mouse IgG1 kappa
Anti-Human gp100 / Melanosome / PMEL17 / SILV Positive Control Sample: SK-MEL-28 cells or Melanoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human SILV protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
gp100 / Melanosome / PMEL17 / SILV Previously Observed Antibody Staining Patterns
| gp100 / Melanosome / PMEL17 / SILV General Information | |
|---|---|
| Alternate Names | |
| Melanocyte protein PMEL, Premelanosome protein, PMEL, Silver locus protein homolog, SILV | |
| Molecular Weight | |
| 90-100kDa | |
| Chromosomal Location | |
| 12q13.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SILV |
| Entrez Gene ID | 6490 |
| Ensemble Gene ID | ENSG00000185664 |
| RefSeq Protein Accession(s) | NP_001186982, XP_006719632, NP_001307050, NP_008859, XP_011536987, NP_001186983, NP_001307051 |
| RefSeq mRNA Accession(s) | XM_006719569, NM_001320122, NM_001200053, XM_011538685, NM_001320121, NM_006928 NM_001200054 |
| RefSeq Genomic Accession(s) | NC_000012, NC_018923, NG_028086 |
| UniProt ID(s) | P40967 |
| UniGene ID(s) | P40967 |
| HGNC ID(s) | 10880 |
| Cosmic ID(s) | PMEL |
| KEGG Gene ID(s) | hsa:6490 |
| PharmGKB ID(s) | PA35781 |
| General Description of gp100 / Melanosome / PMEL17 / SILV. | |
| Cytotoxic T lymphocytes (CTLs) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens, mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S, PMEL 17, is classified as a melanocyte differentiation antigen, is expressed at low levels in normal cell lines, tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein. | |
Limitations and Warranty
enQuire Bio’s gp100 / Melanosome / PMEL17 / SILV Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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