Human and Broad Species Reactivity Anti-GRP94 / HSP90B1 Antibody Product Attributes
GRP94 / HSP90B1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-HSP90B1 antibody staining is expected to be primarily localized to the endoplasmic reticulum.
Observed Antibody Staining Data By Tissue Type:
Variations in GRP94 / HSP90B1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, cells in the molecular layer in cerebellum, cells in the seminiferous ducts in testis, cells in the tubules in kidney, decidual cells in the placenta, endothelial cells in the colon, exocrine glandular cells in the pancreas, fibroblasts in skin, follicle cells in the ovary, germinal center cells in the tonsil, glandular cells in the adrenal gland, appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, seminal vesicle, small intestine, stomach and thyroid gland, glial cells in the caudate nucleus, cerebral cortex and hippocampus, hematopoietic cells in the bone marrow, islets of Langerhans in pancreas, macrophages in lung, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, ovarian stroma cells in the ovary, Purkinje cells in the cerebellum, respiratory epithelial cells in the bronchus and nasopharynx, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, cells in the molecular layer in cerebellum, cells in the seminiferous ducts in testis, cells in the tubules in kidney, decidual cells in the placenta, endothelial cells in the colon, exocrine glandular cells in the pancreas, fibroblasts in skin, follicle cells in the ovary, germinal center cells in the tonsil, glandular cells in the adrenal gland, appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, seminal vesicle, small intestine, stomach and thyroid gland, glial cells in the caudate nucleus, cerebral cortex and hippocampus, hematopoietic cells in the bone marrow, islets of Langerhans in pancreas, macrophages in lung, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, ovarian stroma cells in the ovary, Purkinje cells in the cerebellum, respiratory epithelial cells in the bronchus and nasopharynx, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of GRP94 / HSP90B1 could be seen inmyocytes in skeletal muscle and peripheral nerve in mesenchymal tissue. We were unable to detect GRP94 / HSP90B1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of GRP94 / HSP90B1 expression as measured by anti-GRP94 / HSP90B1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | ++ | +++ | +++ | +++ | +++ | ++ | ++ | +++ | ++ | ++ | ++ | +++ | +++ | +++ | +++ | ++ | +++ | +++ | +++ | ++ |
HSP90B1 Variability | + | ++ | ++ | ++ | + | ++ | ++ | + | ++ | ++ | ++ | + | + | + | + | ++ | ++ | + | + | ++ |
GRP94 / HSP90B1 General Information | |
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Alternate Names | |
Heat shock protein 90kDa beta member 1, HSP90B1, endoplasmin, gp96, grp94, ERp99 | |
Molecular Weight | |
94kDa | |
Chromosomal Location | |
12q23.3 | |
Curated Database and Bioinformatic Data | |
Gene Symbol | HSP90B1 |
Entrez Gene ID | 7184 |
Ensemble Gene ID | ENSG00000166598 |
RefSeq Protein Accession(s) | NP_003290 |
RefSeq mRNA Accession(s) | NM_003299 |
RefSeq Genomic Accession(s) | NC_000012, NC_018923 |
UniProt ID(s) | P14625, V9HWP2 |
UniGene ID(s) | P14625, V9HWP2 |
HGNC ID(s) | 12028 |
Cosmic ID(s) | HSP90B1 |
KEGG Gene ID(s) | hsa:7184 |
PharmGKB ID(s) | PA36705 |
General Description of GRP94 / HSP90B1. | |
Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94), also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94, shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock, are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer. |
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