PDF DatasheetAntibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Lambda Light Chain Antibody Clone: EP172
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Tonsil B Cell Lymphoma
Cellular Localization of Antibody EP172 Staining: Cytoplasmic, cell membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A recombinant protein fragment corresponding to human IgA protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Lambda Light Chain Information for Pathologists
Summary:
Type of immunoglobulin light chain. Kappa/lambda ratio is usually 2:1. Restricted expression of either kappa or lambda suggests monoclonality and a neoplastic process. Microscopic (histologic) images Images hosted on Pathout server:.
| Lambda Light Chain General Information | |
|---|---|
| Alternate Names | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Multiple |
| General Description of Lambda Light Chain . | |
| Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two types of light chains designated as kappa and lambda. The gene rearrangement process that generates the immunoglobulin molecule results in either a productive kappa or lambda gene. The mechanics of the rearrangement process normally produce approximately twice as many kappa-bearing cells as lambda. However this ratio is lost during malignant transformation. The lambda light chain antibody labels the lambda light chain that expresses normal and neoplastic B lymphocytes and plasma cells. Other cells may also express lambdalight chain due to nonspecific uptake of immunoglobulin. Individual B cells express either kappa or lambda light chains. Monoclonality is generally assumed to be evidence of a malignant proliferation. The pairing of a kappa with a lambda light chain antibody is useful for identifying monoclonality of lymphoid malignancies. | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
-150x150.jpg)
-150x150.jpg)
-150x150.jpg)
-150x150.jpg)
-150x150.jpg)
There are no reviews yet.