PDF DatasheetHuman, Rat, Pig, Cow, and Chicken Anti-Neurofilament Antibody Product Attributes
Neurofilament Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-NEFL antibody staining is expected to be primarily localized to the intermediate filaments and nuclear interchromatin granular clusters. There is variability in either the signal strength or the localization of signal in intermediate filaments from cell to cell.
Observed Antibody Staining Data By Tissue Type:
Variations in Neurofilament antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuronal cells in the caudate nucleus, cerebral cortex. More moderate antibody staining intensity was present in neuronal cells in the caudate nucleus, cerebral cortex. Low, but measureable presence of Neurofilament could be seen in cells in the granular layer in cerebellum. We were unable to detect Neurofilament in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| Neurofilament General Information | |
|---|---|
| Alternate Names | |
| Neurofilament light polypeptide, NEFL | |
| Molecular Weight | |
| 68kDa | |
| Chromosomal Location | |
| 8p21.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | NEFL |
| Entrez Gene ID | 4747 |
| Ensemble Gene ID | ENSG00000277586 |
| RefSeq Protein Accession(s) | NP_006149 |
| RefSeq mRNA Accession(s) | NM_006158 |
| RefSeq Genomic Accession(s) | NC_000008, NG_008492, NC_018919 |
| UniProt ID(s) | P07196 |
| UniGene ID(s) | P07196 |
| HGNC ID(s) | 7739 |
| Cosmic ID(s) | NEFL |
| KEGG Gene ID(s) | hsa:4747 |
| PharmGKB ID(s) | PA31542 |
| General Description of Neurofilament. | |
| This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons, dendrites, are found in neurons, peripheral nerves,, sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M), 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine,, endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas,, neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal, extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin,, oat cell carcinomas of the lung also express neurofilament. | |
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