PDF DatasheetHuman Anti-Prostate Specific Antigen Antibody Product Attributes
Species: Human.
Predicted Cross-Reactivity: Feline.
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Prostate Specific Antigen Antibody Clone: 1A7; same as 1A7G6B6
Clone 1A7; same as 1A7G6B6 Host and Isotype: Mouse IgG1 kappa
Anti-Human Prostate Specific Antigen Positive Control Sample: PC12 cells or normal prostate or prostate carcinoma
Cellular Localization of Antibody <1A7; same as 1A7G6B6 Staining: Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: PSA (p30) from human sperm plasma
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Prostate Specific Antigen Previously Observed Antibody Staining Patterns
| Prostate Specific Antigen General Information | |
|---|---|
| Alternate Names | |
| Prostate-specific antigen, PSA, gamma-seminoprotein, kallikrein-3, KLK3 | |
| Molecular Weight | |
| 33-34kDa | |
| Chromosomal Location | |
| 19q13.33 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KLK3 |
| Entrez Gene ID | 354 |
| Ensemble Gene ID | ENSG00000142515 |
| RefSeq Protein Accession(s) | NP_001025218, NP_001025219, NP_001639 |
| RefSeq mRNA Accession(s) | NM_001648, NM_001030047, NM_001030050 NM_001030048, NM_001030049 |
| RefSeq Genomic Accession(s) | NC_018930, NG_011653, NC_000019, |
| UniProt ID(s) | Q546G3, P07288 |
| UniGene ID(s) | Q546G3, P07288 |
| HGNC ID(s) | 6364 |
| Cosmic ID(s) | KLK3 |
| KEGG Gene ID(s) | hsa:354 |
| PharmGKB ID(s) | PA164741810 |
| General Description of Prostate Specific Antigen. | |
| Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA, stains prostatic secretory, ductal epithelium in both normal, neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium,, abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This MAb makes an excellent pair with MAb A67-B/E3 for PSA tests. | |
Limitations and Warranty
enQuire Bio’s Prostate Specific Antigen Anti-Human, Cat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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