PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | No Pretreatment Required |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Prostate Specific Antigen Information for Pathologists
Summary:
Kallikrein related serine protease produced by secretory epithelium, drains into ductal system. Cleaves and liquefies seminal coagulum formed after ejaculation. Clinical features Serum PSA > 4 seen in 80% with histologically documented cancer but also in 25 – 30% with nodular hyperplasia, prostatitis, infarcts, prostatic massage, cystoscopy. Elevated in 2 of 18 post-race marathon runners (Arch Pathol Lab Med 2003;127:345).
Common Uses By Pathologists:
Glycoprotein detected in benign and malignant prostate tissue. Identifies prostatic origin of most metastatic tumors, differentiates between prostatic and urothelial carcinomas. Non-prostate tumors are usually weak / negative for PSA / PAP. PSA is more sensitive (intense, diffuse staining) and specific than PAP. Microscopic (histologic) images
| Prostate Specific Antigen General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 28.7 kDa | |
| Chromosomal Location | |
| q13.33 [chr: 19] [chr_start: 50854915] [chr_end: 50860764] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KLK3 |
| Entrez Gene ID | 354 |
| RefSeq Protein Accession(s) | NP_001025219; NP_001025218; NP_001639 |
| RefSeq mRNA Accession(s) | NM_001648; NM_001030047; NM_001030050; NM_001030048; NM_001030049 |
| RefSeq Genomic Accession(s) | NG_011653; NC_000019; |
| UniProt ID(s) | P07288 |
| PharmGKB ID(s) | PA164741810 |
| KEGG Gene ID(s) | hsa:354 |
| General Description of Prostate Specific Antigen . | |
| This antibody reacts with PSA. The staining was observed in the prostatic ductal epithelium of normal and neoplastic prostate. No staining with any other cell type has been observed. | |
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