PDF DatasheetHuman bFGF / FGF2 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant bFGF / FGF2 based upon sequence from: Human
Host: QP5338 protein expressed in E. coli.
Tag: Untagged
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5338.
Bioactivity Data: BioActivity: Shows bioactivity at a concentration of 5 ng/mL in the BALB/c 3T3 cell proliferation assay. The bioactivity acceptance criteria is set at 2.0 x 10^5 units/mg.
Monomer or Dimer: Monomer
Amino Acid Sequence: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human bFGF / FGF2 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
| Recombinant Human bFGF / FGF2 Protein (Animal Free) General Information | |
|---|---|
| Alternate Names | |
| bFGF, FGF2, FGF-2, HBGF-2, BFGF, FGFB | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | FGF2 |
| Entrez Gene ID | 2247 |
| Ensemble Gene ID | ENSG00000138685 |
| RefSeq Protein Accession(s) | NP_001997.5 |
| RefSeq mRNA Accession(s) | NM_002006.4 |
| UniProt ID(s) | P09038 |
| UniGene ID(s) | Hs.284244 |
| HGNC ID(s) | HGNC:3676 |
| COSMIC ID Link(s) | FGF2 |
| KEGG Gene ID(s) | hsa:2247 |
| PharmGKB ID(s) | PA28115 |
| General Description of Recombinant Human bFGF / FGF2 Protein (Animal Free). | |
| Basic fibroblast growth factor (bFGF), also known as FGF2, is expressed by endothelial cells and is a mediator of angiogenesis. bFGF also has cardioprotective functions during heart injury. The application of bFGF is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Degredation of the full length bFGF N-terminus results in a truncated bFGF147aa protein, when the protein is isolated from biological sources. The N-terminus extensions influence the localization of bFGF within the cell, but do not affect the biological activity of bFGF. Therefore, there are no detectable differences in biological activity between the full length bFGF-154aa and the truncated bFGF-147aa recombinant proteins. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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