PDF DatasheetHuman PDGFA Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PDGFA based upon sequence from: Human
Host: QP5283 protein expressed in E. coli.
Tag: Untagged
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5283.
Bioactivity Data: BioActivity: Shows bioactivity at a concentration of 50 ng/mL in the BALB/c 3T3 cell proliferation assay. The bioactivity acceptance criteria is set at 2.0 x 10^4 units/mg.
Monomer or Dimer: Dimer
Amino Acid Sequence: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human PDGFA Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: Sterile filtered (0.22 um) solution containing 0.1% TFA
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
| Recombinant Human PDGFA Protein General Information | |
|---|---|
| Alternate Names | |
| PDGF1, PDGF-A | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PDGFA |
| Entrez Gene ID | 5154 |
| Ensemble Gene ID | ENSG00000197461 |
| RefSeq Protein Accession(s) | NP_002598.4 |
| RefSeq mRNA Accession(s) | NM_002607.5, NM_033023.4 |
| UniProt ID(s) | P04085 |
| UniGene ID(s) | Hs.535898 |
| HGNC ID(s) | HGNC:8799 |
| COSMIC ID Link(s) | PDGFA |
| KEGG Gene ID(s) | hsa:5154 |
| PharmGKB ID(s) | PA33144 |
| General Description of Recombinant Human PDGFA Protein. | |
| Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-a, TGF-b and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-a and PDGFR-b. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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