PDF DatasheetMouse PDGFA Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PDGFA based upon sequence from Mouse
Host: QP5462 protein expressed in E. coli.
Tag: Untagged
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5462.
Bioactivity Data: 3T3 cell proliferation significantly induced by less than 10 ng/ml of Mouse PDGFA (activity of at least 1.0 x 10^5 units/ml).
Monomer or Dimer: Dimer
Amino Acid Sequence: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: A Certificate of Analysis (CofA) will be sent along with the protein and will contain the proper reconstitution instructions.
Concentration of Mouse PDGFA Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: Sterile filtered (0.22 um) solution containing 0.1% TFA
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
| Recombinant Mouse PDGFA Protein General Information | |
|---|---|
| Alternate Names | |
| PDGF1, PDGF-A | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PDGFA |
| Entrez Gene ID | 18590 |
| RefSeq Protein Accession(s) | NP_032834.1 |
| RefSeq mRNA Accession(s) | NM_008808.3 |
| UniProt ID(s) | P20033 |
| UniGene ID(s) | Mm.2675 |
| COSMIC ID Link(s) | PDGFA |
| KEGG Gene ID(s) | mmu:18590 |
| General Description of Recombinant Mouse PDGFA Protein . | |
| Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by interleukin 1 (IL-1), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF?), transforming growth factor beta (TGF-?), and epidermal growth factor (EGF) signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-a and PDGFR-b. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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