PDF DatasheetHuman Osteonectin / SPARC Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Osteonectin / SPARC based upon sequence from: Human
Host: QP10176 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Human Osteonectin / SPARC, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10176.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ug/mL, corresponding to a specific activity of > 333 IU/mg.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: MSYYHHHHHH DYDIPTTENL YFQGAMGSAP QQEALPDETE VVEETVAEVT
EVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NN
TPMCVC QDPTSCPAPI GEFEKVCSND NKTFDSSCHF FATKCTLEGT KKGH
KLHL DYIGPCKYIP PCLDSELTEF PLRMRDWLKN VLVTLYERDE DNNLLT
EK QKLRVKKIHE NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLD
QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC
FGIKQKDI DKDLVI
Purity: Greater than 95% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human Osteonectin / SPARC Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, pH 7.4, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Osteonectin / SPARC Protein General Information | |
|---|---|
| Alternate Names | |
| Osteonectin; SPARC; BM-40; ON; OI17 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SPARC |
| Entrez Gene ID | 6678 |
| Ensemble Gene ID | ENSG00000113140 |
| RefSeq Protein Accession(s) | NP_001296372.1 |
| RefSeq mRNA Accession(s) | NM_001309443.1, NM_001309444.1, NM_003118.3 |
| UniProt ID(s) | P09486 |
| UniGene ID(s) | Hs.111779 |
| HGNC ID(s) | HGNC:11219 |
| COSMIC ID Link(s) | SPARC |
| KEGG Gene ID(s) | hsa:6678 |
| PharmGKB ID(s) | PA36055 |
| General Description of Recombinant Human Osteonectin / SPARC Protein. | |
| Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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