PDF DatasheetMouse SHH / Sonic hedgehog Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant SHH / Sonic hedgehog based upon sequence from: Mouse
Host: QP10194 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Mouse SHH / Sonic hedgehog, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10194.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by inducing alkaline phosphatase production of murine C3H10T1/2 cells is 0.5 – 1.0 ug/ml.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Purity: Greater than 95% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Mouse SHH / Sonic hedgehog Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered 20 mM PBS, pH 7.4, 150mM NaCl, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse SHH / Sonic hedgehog Protein General Information | |
|---|---|
| Alternate Names | |
| SHH; Sonic hedgehog; HPE3; MCOPCB5; SMMCI; HLP3; TPTPS; HHG1; TPT | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SHH |
| Entrez Gene ID | 20423 |
| Ensemble Gene ID | ENSMUSG00000002633 |
| RefSeq Protein Accession(s) | NP_033196.1 |
| RefSeq mRNA Accession(s) | NM_009170.3 |
| UniProt ID(s) | Q62226 |
| UniGene ID(s) | Mm.57202 |
| COSMIC ID Link(s) | SHH |
| KEGG Gene ID(s) | mmu:20423 |
| General Description of Recombinant Mouse SHH / Sonic hedgehog Protein. | |
| Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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