PDF DatasheetHuman CCL4 / MIP1B Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant CCL4 / MIP1B based upon sequence from: Human
Host: QP10227 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Human CCL4 / MIP1B, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10227.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN
Purity: Greater than 97% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human CCL4 / MIP1B Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, pH 7.4, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human CCL4 / MIP1B Protein General Information | |
|---|---|
| Alternate Names | |
| CCL4; MIP1B; ACT2; HC21; MIP1B1; AT744.1; G-26; MIP1B; SCYA4; LAG1; LAG-1; MIP-1-beta; SCYA2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CCL4 |
| Entrez Gene ID | 388372 |
| Ensemble Gene ID | ENSG00000275302 |
| RefSeq Protein Accession(s) | NP_002975.1 |
| RefSeq mRNA Accession(s) | NM_002984.3, NM_207007.3 |
| UniProt ID(s) | P13236 |
| UniGene ID(s) | Hs.449862 |
| HGNC ID(s) | HGNC:10630 |
| KEGG Gene ID(s) | hsa:388372 |
| PharmGKB ID(s) | PA35563 |
| General Description of Recombinant Human CCL4 / MIP1B Protein. | |
| Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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