PDF DatasheetHuman IL36B / IL1F8 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL36B / IL1F8 based upon sequence from: Human
Host: QP10339 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Human IL36B / IL1F8, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10339.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The specific activity is determined by its binding ability in a functional ELISA. Immobilized rHuIL-36beta at 1 ug/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 ug/mL.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: MNPQREAAPK SYAIRDSRQM VWVLSGNSLI AAPLSRSIKP VTLHLIACRD TEFSDKEKGN MVYLGIKGKD LCLFCAEIQG KPTLQLKEKN IMDLYVEKKA QKPFLFFHNK EGSTSVFQSV SYPGWFIATS TTSGQPIFLT KERGITNNTN FYLDSVE
Purity: Greater than 97% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human IL36B / IL1F8 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, pH 7.4, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human IL36B / IL1F8 Protein General Information | |
|---|---|
| Alternate Names | |
| IL36B; IL1F8; IL-1H2; FIL1; FIL1-(ETA); IL1-ETA; IL1H2; IL-1F8; IL1F8; FIL1H; FILI-(ETA) | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Il36b |
| Entrez Gene ID | 27177 |
| Ensemble Gene ID | ENSG00000136696 |
| RefSeq Protein Accession(s) | NP_055253.2 |
| RefSeq mRNA Accession(s) | NM_014438.4, NM_173178.2, XM_011510962.1 |
| UniProt ID(s) | Q9NZH7 |
| HGNC ID(s) | HGNC:15564 |
| COSMIC ID Link(s) | Il36b |
| KEGG Gene ID(s) | hsa:27177 |
| PharmGKB ID(s) | PA38391 |
| General Description of Recombinant Human IL36B / IL1F8 Protein. | |
| Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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