PDF DatasheetMouse IL36B / IL1F8 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL36B / IL1F8 based upon sequence from: Mouse
Host: QP10437 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Mouse IL36B / IL1F8, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10437.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: SSQSPRNYRV HDSQQMVWVL TGNTLTAVPA SNNVKPVILS LIACRDTEFQ DVKKGNLVFL GIKNRNLCFC CVEMEGKPTL QLKEVDIMNL YKERKAQKAF LFYHGIEGST SVFQSVLYPG WFIATSSIER QTIILTHQRG KLVNTNFYIE SEK
Purity: Greater than 97% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Mouse IL36B / IL1F8 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, pH 7.4, 5% trehalose, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse IL36B / IL1F8 Protein General Information | |
|---|---|
| Alternate Names | |
| IL36B; IL1F8; IL-1H2; FIL1; FIL1-(ETA); IL1-ETA; IL1H2; IL-1F8; IL1F8; FIL1H; FILI-(ETA) | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Il36b |
| Entrez Gene ID | 69677 |
| Ensemble Gene ID | ENSMUSG00000026985 |
| RefSeq Protein Accession(s) | NP_081439.1 |
| RefSeq mRNA Accession(s) | NM_027163.4 |
| UniProt ID(s) | Q9D6Z6 |
| COSMIC ID Link(s) | Il36b |
| KEGG Gene ID(s) | mmu:69677 |
| General Description of Recombinant Mouse IL36B / IL1F8 Protein. | |
| Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensin 4 and beta-defensin 103 as well as a number of matrix metalloproteases (By similarity). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. Induces the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23, and activates p38 MAPK phosphorylation in BMDCs. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by T helper 1 (Th1) cells, cultured CD4(+) T cells and splenocytes. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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