PDF DatasheetMouse IL1F6 / IL36 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL1F6 / IL36 based upon sequence from: Mouse
Host: QP10466 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Mouse IL1F6 / IL36, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10466.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 × 104 IU/mg.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: RAASPSLRHV QDLSSRVWIL QNNILTAVPR KEQTVPVTIT LLPCQYLDTL ETNRGDPTYM GVQRPMSCLF CTKDGEQPVL QLGEGNIMEM YNKKEPVKAS LFYHKKSGTT STFESAAFPG WFIAVCSKGS CPLILTQELG EIFITDFEMI VVH
Purity: Greater than 95% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Mouse IL1F6 / IL36 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, 1 mM DTT, 3 % trehalose, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse IL1F6 / IL36 Protein General Information | |
|---|---|
| Alternate Names | |
| IL1F6; IL36; IL1(EPSILON); FIL1; FIL1(EPSILON); FIL1E; IL-1F6; IL1F6 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Il36a |
| Entrez Gene ID | 54448 |
| Ensemble Gene ID | ENSMUSG00000026984 |
| RefSeq Protein Accession(s) | NP_062323.1 |
| RefSeq mRNA Accession(s) | NM_019450.3 |
| UniProt ID(s) | Q9JLA2 |
| COSMIC ID Link(s) | Il36a |
| KEGG Gene ID(s) | mmu:54448 |
| General Description of Recombinant Mouse IL1F6 / IL36 Protein. | |
| Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. Induces the production of proinflammatory cytokines, including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23 in bone marrow-derived dendritic cells (BMDCs). Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T cells and splenocytes. May play a role in proinflammatory effects in the lung: induces the expression of CXCL1 and CXCL2 in the lung, and the expression of TNF-alpha, IL-36c, IL-1A, IL-1B, CXCL1 and CXCL2 in isolated splenic CD11c(+) alveolar macrophages. May be involved in T cell maturation by stimulating the surface expression of CD40 and modestly CD80 and CD86 in splenic CD11c(+) cells. May be involved in CD4(+) T cell proliferation. Induces NF-kappa B activation in macrophages. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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