PDF DatasheetBovine Alkaline Phosphatase Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Alkaline Phosphatase based upon sequence from Bovine
Host: QP10518 protein expressed in Native Protein.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Alkaline Phosphatase was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10518.
Bioactivity Data: >1000 U/mg (pH 9.6, 25°C and 0.025M glycin, 10% glycerol as buffer.
Monomer or Dimer: Dimer
Amino Acid Sequence: MQGACVLLLL GLHLQLSLGL VPVEEEDPAF WNRQAAQALD VAKKLQPIQT AAKNVILFLG DGMGVPTVTA TRILKGQMNG KLGPETPLAM DQFPYVALSK TYNVDRQVPD SAGTATAYLC GVKGNYRTIG VSAAARYNQC KTTRGNEVTS VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADLPADAQMN GCQDIAAQLV NNMDIDVILG GGRKYMFPVG TPDPEYPDDA SVNGVRKRKQ NLVQAWQAKH QGAQYVWNRT ALLQAADDSS VTHLMGLFEP ADMKYNVQQD HTKDPTLQEM TEVALRVVSR NPRGFYLFVE GGRIDHGHHD DKAYMALTEA GMFDNAIAKA NELTSELDTL ILVTADHSHV FSFGGYTLRG TSIFGLAPSK ALDSKSYTSI LYGNGPGYAL GGGSRPDVND STSEDPSYQQ QAAVPQASET HGGEDVAVFA RGPQAHLVHG VEEETFVAHI MAFAGCVEPY TDCNLPAPTT ATSIPDAAHL AASPPPLALL AGAMLLLLAP TLY
Purity: 95% pure by Gel Filtration.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: 50% glycerol, 5mM MgCl2, 0.1mM ZnCl2 and 5mM TRIS, pH 7.0.
Storage Conditions: AP should be stored at 4°C. Please Do-Not freeze.
Physical Appearance: Sterile Filtered clear colorless solution.
| Recombinant Bovine Alkaline Phosphatase General Information | |
|---|---|
| Alternate Names | |
| EC 3.1. 3.1, IAP, AP, ALPI, Intestinal-type alkaline phosphatase, Intestinal alkaline phosphatase. | |
| Molecular Weight | |
| 140 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P19111 |
| General Description of Recombinant Bovine Alkaline Phosphatase. | |
| The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity. Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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