PDF DatasheetPorcine IL 1 alpha Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL 1 alpha based upon sequence from Porcine
Host: QP10685 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IL 1 alpha was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Interleukin-1 alpha in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by the dose-dependant stimulation of D10S cells is < 0.03 ng/ml.
Amino Acid Sequence: MAKVPDLFED LKNCYSENEE YSSDIDHLSL NQKSFYDASY EPLPGDGMDK FMPLSTSKTS KTSRLNFKDS VVMAAANGKI LKKRRLSLNQ FITDDDLEAI ANDTEEEIIK PRSATYSFQS NMKYNFMRVI NHQCILNDAR NQSIIRDPSG QYLMAAVLNN LDEAVKFDMA AYTSNDDSQL PVTLRISETR LFVSAQNEDE PVLLKELPET PKTIKDETSL LFFWEKHGNM DYFKSAAHPK LFIATRQEKL VHMAPGLPSV TDFQILENQS
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Porcine IL 1 alpha Protein: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.669 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-1 as a Reference Standard.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing no additives.
Storage Conditions: Lyophilized Interleukin-1 alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Porcine IL 1 alpha General Information | |
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| Alternate Names | |
| Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha, IL1, IL-1A, IL1F1. | |
| Molecular Weight | |
| 18.1 kDa | |
| Chromosomal Location | |
| On chromosome 3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IL1A |
| Entrez Gene ID | 397094 |
| UniProt ID(s) | P18430 |
| COSMIC ID Link(s) | IL1A |
| KEGG Gene ID(s) | ssc:397094 |
| General Description of Recombinant Porcine IL 1 alpha. | |
| Interleukin-1A Porcine Recombinant produced in E. Coli is single, a non-glycosylated, Polypeptide chain containing 158 amino acids and having a molecular mass of 18076 Dalton. The IL-1A is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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