PDF DatasheetRat IL 1 alpha Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL 1 alpha based upon sequence from Rat
Host: QP10686 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IL 1 alpha was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Interleukin 1a in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by the dose-dependant stimulation of murine D10S cells is < 0.005 ng/ml, corresponding to a Specific Activity of 200,000,000IU/mg.
Amino Acid Sequence: MAKVPDLFED LKNCYSENEE YSSAIDHLSL NQKSFYDASY GSLHENCTDK FVSLRTSETS KMSTFTFKES RVVVSATSNK GKILKKRRLS FNQPFTEDDL EAIAHDLEET IQPRSAPHSF QNNLRYKLIR IVKQEFIMND SLNQNIYVDM DRIHLKAASL NDLQLEVKFD MYAYSSGGDD SKYPVTLKVS NTQLFVSAQG EDKPVLLKEI PETPKLITGS ETDLIFFWEK INSKNYFTSA AFPELLIATK EQSQVHLARG LPSMIDFQIS
Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing 50mM Tris-HCl, pH=8.
Storage Conditions: Lyophilized Interleukin-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rat IL 1 alpha General Information | |
|---|---|
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| Alternate Names | |
| Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1. | |
| Molecular Weight | |
| 17.7 kDa | |
| Chromosomal Location | |
| q36 on chromosome 3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Il1a |
| Entrez Gene ID | 24493 |
| UniProt ID(s) | P16598 |
| COSMIC ID Link(s) | Il1a |
| KEGG Gene ID(s) | rno:24493 |
| General Description of Recombinant Rat IL 1 alpha. | |
| Rat Interleukin-1A produced in E. Coli is single, a non-glycosylated, Polypeptide chain containing 155 amino acids and having a molecular mass of 17703 Dalton. The IL-1A is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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