PDF DatasheetRat IL 1 alpha Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant IL 1 alpha based upon sequence from Rat
Host: QP12390 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of IL 1 alpha was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12390.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSAPHSFQ NNLRYKLIRI VKQEFIMNDS LNQNIYVDMD RIHLKAASLN DLQLEVKFDM YAYSSGGDDS KYPVTLKVSN TQLFVSAQGE DKPVLLKEIP ETPKLITGSE TDLIFFWEKI NSKNYFTSAA FPELLIATKE QSQVHLARGL PSMIDFQIS
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: IL 1 alpha protein solution (1 mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Rat IL 1 alpha General Information | |
|---|---|
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| Alternate Names | |
| Interleukin-1 alpha, IL-1 alpha. | |
| Molecular Weight | |
| 20.2 kDa | |
| Chromosomal Location | |
| q36 on chromosome 3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Il1a |
| Entrez Gene ID | 24493 |
| UniProt ID(s) | P16598 |
| COSMIC ID Link(s) | Il1a |
| KEGG Gene ID(s) | rno:24493 |
| General Description of Recombinant Rat IL 1 alpha. | |
| Rat IL 1 alpha produced in E. Coli is a single, non-glycosylated polypeptide chain containing 179 amino acids (115-270 AA) and having a molecular mass of 20.2kDa. IL 1 alpha is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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