PDF DatasheetRat IL 15 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL 15 based upon sequence from Rat
Host: QP10693 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IL 15 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Interleukin-15 in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 10 ng/ml, corresponding to a Specific Activity of 100,000IU/mg.
Amino Acid Sequence: MKILKPYMRN TSILYYLCFL LNSHFLTEAG IHVFILGCVS VGLPKTEANW IDVRYDLEKI ESLIQFIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH EYSNMTLNET VRNVLYLANS TLSSNKNVIE SGCKECEELE ERNFTEFLQS FIHIVQMFIN TS
Purity: Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from 10mM Tris, pH 8.5.
Storage Conditions: Lyophilized Interleukin-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL15 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rat IL 15 General Information | |
|---|---|
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| Alternate Names | |
| IL-15, MGC9721. | |
| Molecular Weight | |
| 13.5 kDa | |
| Chromosomal Location | |
| q11 on chromosome 19 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Il15 |
| Entrez Gene ID | 25670 |
| UniProt ID(s) | P97604 |
| COSMIC ID Link(s) | Il15 |
| KEGG Gene ID(s) | rno:25670 |
| General Description of Recombinant Rat IL 15. | |
| Rat Interleukin-15 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 13533 Dalton. The IL-15 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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