PDF DatasheetMouse LDHA Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant LDHA based upon sequence from Mouse
Host: QP10743 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of LDHA was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10743.
Bioactivity Data: Specific activity is > 250 units/mg, and is defined as the Amount of enzyme that convert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37C.
Amino Acid Sequence: MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QFLEHHHHHH
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: LDHA protein solution (0.5 mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Mouse LDHA General Information | |
|---|---|
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| Alternate Names | |
| L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M. | |
| Molecular Weight | |
| 37.5 kDa | |
| Chromosomal Location | |
| B3 on chromosome 7 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Ldha |
| Entrez Gene ID | 16828 |
| UniProt ID(s) | P06151 |
| COSMIC ID Link(s) | Ldha |
| KEGG Gene ID(s) | mmu:16828 |
| General Description of Recombinant Mouse LDHA. | |
| Mouse LDHA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 340 amino acids (1-332AA ) and having a molecular mass of 37.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). LDHA is expressed with an 8 amino acid His tag at C-Terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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