PDF DatasheetRat LDHA Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant LDHA based upon sequence from Rat
Host: QP12549 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of LDHA was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12549.
Bioactivity Data: Untested
Amino Acid Sequence: MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: LDHA protein solution (0.25 mg/ml) containing Phosphate Buffered Saline (pH 7.4), 1mM DTT and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Rat LDHA General Information | |
|---|---|
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| Alternate Names | |
| L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M. | |
| Molecular Weight | |
| 37.5 kDa | |
| Chromosomal Location | |
| q22 on chromosome 1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Ldha |
| Entrez Gene ID | 24533 |
| UniProt ID(s) | P04642 |
| COSMIC ID Link(s) | Ldha |
| KEGG Gene ID(s) | rno:24533 |
| General Description of Recombinant Rat LDHA. | |
| Rat LDHA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 340 amino acids (1-332 AA) and having a molecular mass of 37.5kDa (Migrates at 28-40kDa on SDS-PAGE under reducing conditions). LDHA is fused to an 8 amino acid His-tag at C-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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